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Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis
 
Creator Patidar , Anil
Malhotra, Himanshu
Chaudhary, Surbhi
Kumar, Manoj
Dilawari, Rahul
Chaubey, Gaurav Kumar
Dhiman, Asmita
Modanwal , Radheshyam
Talukdar, Sharmila
Raje , Chaaya Iyengar
Raje, Manoj
 
Subject QR Microbiology
 
Description Availability of iron is a key factor in the survival and multiplication of Mycobacterium tuberculosis (M.tb) within host macrophage phagosomes. Despite host cell iron regulatory machineries attempts to deny supply of this essential micronutrient, intraphagosomal M.tb continues to access extracellular iron. In the current study, we report that intracellular M.tb exploits mammalian secreted Glyceraldehyde 3-phosphate dehydrogenase (sGAPDH) for the delivery of host iron carrier proteins lactoferrin (Lf) and transferrin (Tf). Studying the trafficking of iron carriers in infected cells we observed that sGAPDH along with the iron carrier proteins are preferentially internalized into infected cells and trafficked to M.tb containing phagosomes where they are internalized by resident mycobacteria resulting in iron delivery. Collectively our findings provide a new mechanism of iron acquisition by M.tb involving the hijack of host sGAPDH. This may contribute to its successful pathogenesis and provide an option for targeted therapeutic intervention.
 
Publisher Springer
 
Date 2022
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s00018-021-04110-3
http://crdd.osdd.net/open/2989/
 
Identifier Patidar , Anil and Malhotra, Himanshu and Chaudhary, Surbhi and Kumar, Manoj and Dilawari, Rahul and Chaubey, Gaurav Kumar and Dhiman, Asmita and Modanwal , Radheshyam and Talukdar, Sharmila and Raje , Chaaya Iyengar and Raje, Manoj (2022) Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis. Cellular and Molecular Life Sciences , 79 (1).