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Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica
 
Creator Svec, Pavel
Kralova, Stanislava
Stankova, Eva
Holochova, Pavla
Sedlar, Karel
Koudelkova, Sylva
Krsek, Daniel
Grzesiak, Jakub
Sedo, Ondrej
Vaczi, Peter
Urvashi, .
Gupta, Vipin
Sood , Utkarsh
Lal, Rup
Korpole, Suresh
Sedlacek, Ivo
 
Subject QR Microbiology
 
Description Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA-DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T).
 
Publisher International Union of Microbiological Societies
 
Date 2022-04
 
Type Article
PeerReviewed
 
Relation https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005309#tab2
http://crdd.osdd.net/open/2995/
 
Identifier Svec, Pavel and Kralova, Stanislava and Stankova, Eva and Holochova, Pavla and Sedlar, Karel and Koudelkova, Sylva and Krsek, Daniel and Grzesiak, Jakub and Sedo, Ondrej and Vaczi, Peter and Urvashi, . and Gupta, Vipin and Sood , Utkarsh and Lal, Rup and Korpole, Suresh and Sedlacek, Ivo (2022) Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica. International journal of systematic and evolutionary microbiology, 72 (4). ISSN 1466-5034