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<p><strong>Ayurvedic, herbal extracts suppress Candidal biofilms <em>in vitro</em></strong></p>

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Title Statement <p><strong>Ayurvedic, herbal extracts suppress Candidal biofilms <em>in vitro</em></strong></p>
 
Added Entry - Uncontrolled Name Wijesinghe, Gayan Kanchana
Jayarathna, Thilini Anupama
Gunasekara, Chinthika
Fernando, Neluka
Kottegoda, Nilwala
Samaranayake, Lakshman
Weerasekera, Manjula Manoji
 
Summary, etc. <p>Plant derivatives have been used for centuries to treat various human afflictions including microbial infections. A vast majority of these infections are initiated and perpetuated by community dwelling, surface-attached organisms living in micro-econiches known as biofilms. We investigated the biofilm suppressant effect of phytomedicinal preparations used widely in traditional Ayurvedic medicine, Triphala, a mixture of <em>Terminalia bellirica</em>, <em>Terminalia chebula</em> and <em>Emblica officinalis</em>, and <em>Mimusops elengi </em>bark extract. Inhibitory effect of extracts<em> </em>were first investigated against the planktonic <em>C. albicans </em>and <em>C. tropicalis</em> using the well diffusion. Minimum biofilm inhibitory concentration for <em>in-vitro</em> biofilms was determined by MTT assay. The biofilm suppressant effect was determined by measuring biofilm viability at different time intervals, post-exposure to the two herbal extracts, and using MTT. Scanning electron microscopy was performed to assess the post-exposure biofilm architecture. Triphala inhibited both species of the planktonic yeasts, and only the biofilm phase <em>C. tropicalis </em>and mixed species<em>, </em>and not<em> C. albicans. M. elengi </em>had no inhibitory effect on either the planktonic or the biofilms of either <em>Candida</em> species. Ultrastructural microscopy revealed increased cell density of <em>C. albicans</em> biofilm, but not that of <em>C. tropicalis</em> which was significantly reduced in size after Triphala exposure. Triphala, but not <em>M. elengi, </em>extracts exhibit selective and differential biofilm inhibitory activity against <em>Candida</em>. <em>C. albicans </em>biofilms are more resistant to the anti-biofilm activity of Triphala.<em></em></p>
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2022-07-26 07:24:30
 
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http://op.niscair.res.in/index.php/IJTK/article/view/46000
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 21, ##issue.no## 3 (2022): Indian Journal of Traditional Knowledge
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJTK/article/download/46000/465547585
http://op.niscair.res.in/index.php/IJTK/article/download/46000/465547586
http://op.niscair.res.in/index.php/IJTK/article/download/46000/465547587
http://op.niscair.res.in/index.php/IJTK/article/download/46000/465547588
http://op.niscair.res.in/index.php/IJTK/article/download/46000/465547589
http://op.niscair.res.in/index.php/IJTK/article/download/46000/465547590
http://op.niscair.res.in/index.php/IJTK/article/download/46000/465547600