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Effects of heavy metals on the viability of A549 cells

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Field Value
 
Authentication Code dc
 
Title Statement Effects of heavy metals on the viability of A549 cells
 
Added Entry - Uncontrolled Name Qian-wen, Wang
Jae-sik, Jeon
Jae Kyung, Kim
 
Uncontrolled Index Term Cytokine; Lactate dehydrogenase; Particulate matter
 
Summary, etc. Heavy metals, such as Cd and Pb, are present in the particulate matter (PM) suspended in ambient air. Once internalized, they often accumulate in the human body and cannot be metabolized for long periods. Most previous studies on Cd and Pb metabolism used high concentrations of these heavy metals. In this study, we analysed the effects of low concentrations of Cd or Pb (similar to their concentrations in the PM) on the viability of lung epithelial cells and the secretion of pro-inflammatory factors, IL-6 and IL-8. A549 cells were cultured in the presence of Cd and Pb at 1 µg/L and 1 mg/L for 0, 4, 12, and 24 h and analysed using WST-8 and lactate dehydrogenase (LDH) assays and ELISA. Our results revealed that Cd and Pb induced comparable cytotoxicity and secretion of pro-inflammatory cytokines, IL-6 and IL-8, in a time-dependent manner, even at low concentrations. Altogether, our results suggest that heavy metals present in PM can cause chronic long-term effects on human lungs.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2022-07-29 13:41:38
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJBB/article/view/62639
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); IJBB Vol. 59 (7) [July 2022]
 
Language Note en