Record Details

Cloning and characterization of phloroglucinol biosynthetic gene <em>phl</em>C from an Indian strain of <em>Pseudomonas fluorescens</em>

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Cloning and characterization of phloroglucinol biosynthetic gene <em>phl</em>C from an Indian strain of <em>Pseudomonas fluorescens</em>
 
Added Entry - Uncontrolled Name Gupta, Payal ; ICAR-NIPB
Karthik, Kesiraju ; ICAR-NIPB
Sreevathsa, Rohini ; ICAR-NIPB
Rai, Rhitu ; ICAR-NIPB
Dash, Prasanta K; ICAR
ICAR, New Delhi
 
Uncontrolled Index Term PGPR; phlABCD; Phloroglucinol; Polyketide; Protein model database (PMDB); Ramchandran plot
 
Summary, etc. <p style="text-align: justify;">Phloroglucinol or 2,4-diacetyl phloroglucinol (DAPG) is a polyketide compound produced by gram negative soil bacteria <em>Pseudomonas</em>. It shows broad spectrum antibacterial and antifungal properties against soil-borne plant pathogens. In <em>Pseudomonas</em> spp., genes for biosynthesis of 2,4-DAPG are localized in <em>phl</em>ABCD operon. All the four genes in <em>phl</em>ABCD operon are indispensable and DAPG synthesis is attenuated even in the absence of one of the genes. In the present study, we identified and cloned <em>phl</em>C gene from an Indian strain of <em>Pseudomonas</em> and analyzed its sequence. The structural details ofthe PHLC protein was generated by three-dimensional homology modelling. Additionally, stereo-chemical properties of PHLC were analyzed by Ramachandran plot analysis and the generated model was validated by PDBsum. Our results demonstrate that the cloned PHLC protein contains structural features typical of a condensing enzyme involved inpolyketide synthesis.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2022-08-01 00:00:00
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/48629
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 60, ##issue.no## 08 (2022): IJEB [AUGUST 2022]
 
Language Note en
 
Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJEB/article/download/48629/465557568
http://op.niscair.res.in/index.php/IJEB/article/download/48629/465557569
http://op.niscair.res.in/index.php/IJEB/article/download/48629/465557570
http://op.niscair.res.in/index.php/IJEB/article/download/48629/465557571
http://op.niscair.res.in/index.php/IJEB/article/download/48629/465557572
http://op.niscair.res.in/index.php/IJEB/article/download/48629/465557573