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Qualitative assessment of bioactive compounds of actinomycetes AIA25a isolate using HPTLC and GCMS technique

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Title Qualitative assessment of bioactive compounds of actinomycetes AIA25a isolate using HPTLC and GCMS technique
 
Creator Kumari, Nalinee
Menghani, Ekta
Kumawat, Tarun Kumar
Vashishtha, Abhishek
 
Subject Actinomycetes
AIA25a
Bioautography
Florescent light
HPTLC
Partial purification
 
Description 303-310
In the present study AIA25a strain has been isolated from the rhizospheric soil actinomycetes from selected locations
of Rajasthan. Antimicrobial activity test of AIA25a against bacterial strains S. aureus (MTCC 3160), P. aeruginosa
(MTCC 1688), P. mirabilis (ITCC 425) has been carried out. A good activity is shown by chloroform crude extract against
P. aeruginosa (IZ=13.4±0.3mm), moderate activity (IZ=11.1±0.1) is shown against S. aureus and no activity is observed
against P. mirabilis strain. Different compounds isolation is executed with the help of chloroform solvent. Using GC-MS
identification has been carried out for compounds present in AIA25a strain followed by partial purification of compounds.
With the help of HPTLC method partial purification of compounds has been achieved and followed by GC-MS analysis for
identification of separated compounds. The Bioautography technique has been applied for the detection of antimicrobial spot
of partial purified compounds of crude extract of AIA25a isolate. Results of this study show the presence of various
compounds in the rhizospheric soil of Rajasthan.
 
Date 2022-06-27T09:19:45Z
2022-06-27T09:19:45Z
2022-05
 
Type Article
 
Identifier 0975-0991 (Online); 0971-457X (Print)
http://nopr.niscpr.res.in/handle/123456789/59931
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJCT Vol.29(3) [May 2022]