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Modified protocol to overcome in vitro recalcitrance of premature micro-shoots abscission by silver supplementation in Kinnow mandarin (Citrus nobilis L. × Citrus deliciosa T.)

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Title Modified protocol to overcome in vitro recalcitrance of premature micro-shoots abscission by silver supplementation in Kinnow mandarin (Citrus nobilis L. × Citrus deliciosa T.)
 
Creator Murugan, Theivanai
Awasthi, Om Prakash
Singh, Sanjay Kumar
Dubey, Anil Kumar
Kumar, Kanika
 
Subject GA3
Hardwood
MS medium
Nodal segments
 
Description 687-695
The present study focused on alleviating the issue of premature abscission of micro-shoots emerged from the cultured
nodal segments in Kinnow mandarin. To resolve the problem, explants of different maturity stages, culture media, growth
regulator combinations and ethylene inhibitors (silver compounds) were evaluated. Hardwood stage was observed to be the
most efficient explant maturity stage to boost the vigorous early shoot emergence on MS (Murashige and Skoog) medium.
Addition of GA3 (Gibberellic acid) 10 mg L−1 resulted in delayed abscission. Further reduction in abscission rate and healthy
shoot growth were observed when subculturing of micro-shoots was done on MS medium supplemented with
BAP 2.5 mg L−1, GA3 10 mg L−1 and silver thiosulfate 5 mg L−1. This modified procedure could be effectively utilized for
mass multiplication as well as crop improvement in Kinnow mandarin.
 
Date 2022-07-01T10:57:03Z
2022-07-01T10:57:03Z
2022-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60007
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.59(6) [June 2022]