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Immunocytochemical evidence of histamine 1 and histamine 2 receptors on mice sperm

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Title Immunocytochemical evidence of histamine 1 and histamine 2 receptors on mice sperm
 
Creator AzimahAhmad@Aziz, Nor
Hashim, Noor Hashida
Yusuf, Yusmin Mohd
 
Subject Histamine receptors
Immunocytochemistry
Localisation
Sperm
 
Description 632-639
Histamine is a biogenic amine which is synthesised by L-histidine decarboxylase enzyme (HDC). The histamine 1 and 2 antagonist administrations have been highly reported to cause detrimental effect on sperm parameters, which arisen the speculation of histamine 1 (H1R) and histamine 2 (H2R) receptors might be present in sperm. The present study was aimed to provide evidence on the localisation of H1R and H2R on mice sperm through immunocytochemistry. The sperm was harvested from cauda epididymis. After one hour of incubation, sperm suspension was smeared onto a poly-lysine-coated slide and allowed to dry before fixation and permeabilisation processes. The primary antibody encoded for receptors was exposed to the fluorescently tagged antibody; fluorescein isothiocyanate (FITC) conjugate followed by nuclear staining with 4, 6-diamino-2-phenylindole dihydrochloride (DAPI). The testis, stomach, and skin were used as the positive controls. Our data showed that both receptors have been expressed on the midpiece and acrosome of mice. The present result was the first discovery of the presence and immunolocalisation of H1R and H2R on mice sperm. Therefore, present study proposes that these receptors could be involved in calcium regulatory mechanism and protein phosphorylation which are responsible for fertilisation-related processes.
 
Date 2022-07-01T11:11:31Z
2022-07-01T11:11:31Z
2022-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60012
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.59(6) [June 2022]