Antiproliferative potential of Sargassum binderi Sonder ex J. Agardh mediated biogenic silver/silver chloride nanoparticles on EAC, MCF-7 and HCT116 cells
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Title |
Antiproliferative potential of Sargassum binderi Sonder ex J. Agardh mediated biogenic silver/silver chloride nanoparticles on EAC, MCF-7 and HCT116 cells
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Creator |
Ahamed, M S
Rahman, M M Hasan, I Kabir, S R Asaduzzaman, A K M |
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Subject |
Ag/AgCl-NPs
Apoptosis Cancer cell inhibition Gene expression Sargassum binderi |
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Description |
126-137
Biosynthesis of Ag/AgCl-NPs from Sargassum binderi extract was confirmed by colour changes and a sharp peak at 416 nm detected by UV-visible spectrophotometer. It was characterized by Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX) spectrophotometer, X-ray powder Diffraction (XRD) and Fourier-Transform Infrared Spectroscopy (FTIR). Mild toxicity of the synthesized Ag/AgCl-NPs was determined by the brine shrimp nauplii cytotoxicity assay. IC50 values of 19.73, 25.56, and 14.00 𝜇g/ml showed the growth inhibition of Ehrlich Ascites Carcinoma (EAC), human breast cancer (MCF-7) and human colon cancer (HCT116) cells by Ag/AgCl-NPs, respectively. By analyzing cell morphological study using annexin V and caspase-3 inhibitor, initiation of apoptosis in EAC cells was confirmed. The results were further supported through the expression of several genes, where, p53 and Bax genes became activated and Bcl-X and NFκB genes were suppressed. |
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Date |
2022-07-01T11:48:51Z
2022-07-01T11:48:51Z 2022-02 |
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Identifier |
2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/60024 |
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Language |
en
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Publisher |
NIScPR-CSIR, India
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Source |
IJMS Vol.51(02) [February 2022]
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