Forkhead box A1 expression in thyroid carcinoma based on bioinformatics and clinical significance
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Title |
Forkhead box A1 expression in thyroid carcinoma based on bioinformatics and clinical significance
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Creator |
Wang, Jinglu
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Subject |
Hepatocyte nuclear factor
Thyroid gland Tumor |
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Description |
521-526
Thyroid cancer, known to be common in women than men, accounts for 12% all types of cancers and ranks 9th with 5,86,202 cases worldwide. Role of forkhead box A1 (FOXA1), also known as hepatocyte nuclear factor 3α and involved in the oncogenesis and progression of several tumors such as gliomas, breast, stomach, lung, ovarian, and esophageal cancers, has not been elucidated well in thyroid carcinoma until now. Here, we analyzed the expression of FOXA1 in thyroid carcinoma tissues and its effects on the biological characteristics of papillary thyroid carcinoma TPC-1 cells. The expression levels of FOXA1 in normal thyroid and thyroid carcinoma tissues were analyzed using UALCAN database (http://ualcan.path.uab.edu/index.html), and the correlations between FOXA1 expression levels and survival time of patients with thyroid carcinoma were analyzed. Si-FOXA1 and si-NC were transfected into cells that were then divided into si-FOXA1 (transfected si-FOXA1), si-NC (transfected si-NC), and control (TPC-1) groups. Cell proliferation was determined with MTT assay, and invasion ability was measured by Transwell assay. Early apoptotic rate was detected by flow cytometry. The mRNA expression levels of p27Kip1, Cyclin D1 and Cyclin E were measured by qRT-PCR. The expression level of FOXA1 was significantly higher in thyroid carcinoma tissues than that in normal thyroid tissues. UALCAN-based analysis indicated that patients with low expression level of FOXA1 had longer survival time than those with high expression level of FOXA1 (P |
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Date |
2022-07-11T07:26:35Z
2022-07-11T07:26:35Z 2022-07 |
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Type |
Article
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Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/60083 |
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Language |
en
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Publisher |
NIScPR-CSIR, India
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Source |
IJEB Vol.60(07) [July 2022]
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