Record Details

Biomimetic of hydroxyapatite with Tridax procumbens leaf extract and investigation of antibiofilm potential in Staphylococcus aureus and Escherichia coli

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Biomimetic of hydroxyapatite with Tridax procumbens leaf extract and investigation of antibiofilm potential in Staphylococcus aureus and Escherichia coli
 
Creator Nagaraj, Anusuya
Kalagatur, Naveen Kumar
Kadirvelu, Krishna
Shankar, Sushmitha
Mangamuri, Usha Kiranmayi
Sudhakar, P
Samiappan, Suja
 
Subject Antioxidant activity
Biocomposite
Biofilm
Hydroxyapatite
Tridax procumbens
 
Description 755-766
In the last few decades, hydroxyapatite (HA) has become one of the most highly priced biominerals in the biomedical industry for orthopedic and dental applications. The focus of this research was to synthesize biomimetic HA from Tridax procumbens (TP) leaf extract and investigate their antibiofilm properties. The HA was made using the sol-gel method and the HA-TP biocomposite was made by precipitation method. The d.nm size of HA and HA-TP biocomposite was determined as 193.28 and 258.14 d.nm, respectively. The zeta potential of HA and HA-TP biocomposite was determined as −21.2 and −18.3 mV, respectively, and found highly stable. The FTIR study revealed that phytochemicals of TP weresuccessfully impregnated into HA-TP biocomposite. The HA and HA-TP biocomposite were found spherical andagglomerated from SEM analysis. In HR-TEM analysis, the average diameter of the HA and HA-TP biocomposite were16.57 – 64.22 nm and 51.71 – 138.68 nm, respectively. According to the EDX analysis, HA is primarily composed ofcalcium, oxygen, and phosphate, whereas, HA-TP biocomposite is primarily composed of calcium, phosphate, oxygen, andcarbon. In the antioxidant assay, the IC50 value (concentration required to scavenge 50% of free radicals) of HA-TPbiocomposite was determined as 156.69 ± 14.02 and 180.21 ± 12.84 μg/mL in DPPH and ABTS free radical scavengingassays, respectively. The MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) of as-synthesized HA-TP biocomposite against Staphylococcus aureus – ATCC 13565 and Escherichia coli – MTCC 41 wereobserved as 181.09 ± 21.47 and 317.30 ± 41.03, and 157.59 ± 32.18 and 264.03 ± 21.58 μg/mL, respectively. The as-synthesized HA-TP biocomposite has detrimentally affected the biofilm formation of both the tested bacteria S. aureus –ATCC 13565 and E. coli – MTCC 41. The study concluded that the as-synthesized HA-TP biocomposite could be highlyhelpful in the biomedical field for alleviating oxidative-stress-related disorders and inhibiting microbial biofilm formation.
 
Date 2022-08-01T06:41:59Z
2022-08-01T06:41:59Z
2022-07
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60224
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.59(7) [July 2022]