Biomimetic of hydroxyapatite with Tridax procumbens leaf extract and investigation of antibiofilm potential in Staphylococcus aureus and Escherichia coli
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Title |
Biomimetic of hydroxyapatite with Tridax procumbens leaf extract and investigation of antibiofilm potential in Staphylococcus aureus and Escherichia coli
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Creator |
Nagaraj, Anusuya
Kalagatur, Naveen Kumar Kadirvelu, Krishna Shankar, Sushmitha Mangamuri, Usha Kiranmayi Sudhakar, P Samiappan, Suja |
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Subject |
Antioxidant activity
Biocomposite Biofilm Hydroxyapatite Tridax procumbens |
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Description |
755-766
In the last few decades, hydroxyapatite (HA) has become one of the most highly priced biominerals in the biomedical industry for orthopedic and dental applications. The focus of this research was to synthesize biomimetic HA from Tridax procumbens (TP) leaf extract and investigate their antibiofilm properties. The HA was made using the sol-gel method and the HA-TP biocomposite was made by precipitation method. The d.nm size of HA and HA-TP biocomposite was determined as 193.28 and 258.14 d.nm, respectively. The zeta potential of HA and HA-TP biocomposite was determined as −21.2 and −18.3 mV, respectively, and found highly stable. The FTIR study revealed that phytochemicals of TP weresuccessfully impregnated into HA-TP biocomposite. The HA and HA-TP biocomposite were found spherical andagglomerated from SEM analysis. In HR-TEM analysis, the average diameter of the HA and HA-TP biocomposite were16.57 – 64.22 nm and 51.71 – 138.68 nm, respectively. According to the EDX analysis, HA is primarily composed ofcalcium, oxygen, and phosphate, whereas, HA-TP biocomposite is primarily composed of calcium, phosphate, oxygen, andcarbon. In the antioxidant assay, the IC50 value (concentration required to scavenge 50% of free radicals) of HA-TPbiocomposite was determined as 156.69 ± 14.02 and 180.21 ± 12.84 μg/mL in DPPH and ABTS free radical scavengingassays, respectively. The MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) of as-synthesized HA-TP biocomposite against Staphylococcus aureus – ATCC 13565 and Escherichia coli – MTCC 41 wereobserved as 181.09 ± 21.47 and 317.30 ± 41.03, and 157.59 ± 32.18 and 264.03 ± 21.58 μg/mL, respectively. The as-synthesized HA-TP biocomposite has detrimentally affected the biofilm formation of both the tested bacteria S. aureus –ATCC 13565 and E. coli – MTCC 41. The study concluded that the as-synthesized HA-TP biocomposite could be highlyhelpful in the biomedical field for alleviating oxidative-stress-related disorders and inhibiting microbial biofilm formation. |
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Date |
2022-08-01T06:41:59Z
2022-08-01T06:41:59Z 2022-07 |
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Type |
Article
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Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60224 |
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Language |
en
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Publisher |
NIScPR-CSIR,India
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Source |
IJBB Vol.59(7) [July 2022]
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