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Decolorization of anthraquinone based dye (Vat Brown R) by Pseudomonas aeruginosa NCH - Optimization and kinetic study

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Title Decolorization of anthraquinone based dye (Vat Brown R) by Pseudomonas aeruginosa NCH - Optimization and kinetic study
 
Creator Mohanty, Swati Sambita
Kumar, Arvind
 
Subject Biodecolourization
Kinetics
Optimization
Pseudomonas aeruginosa NCH
Response surface methodology
Vat Brown R
 
Description 709-717
The objective of this research work is related to the fact that the source of isolation and acclimatization process influences
the microorganism’s potential for the decolorization of various substances. Some of the widely used anthraquinone vat dyes
decolorization by the pure bacterial strain is a significant aspect that will assist in the in-situ bioremediation of the
ecosystem.The present study is to evaluate the enhanced decolorization of Vat Brown R by an isolated bacterium, Pseudomonas
aeruginosa NCH, from textile dye wastewater under aerobic conditions. The effect of pH, temperature, and inoculum size was
optimized using response surface methodology with the box-behnken experimental design. The strain NCH showed maximum
decolorization efficacy under optimum conditions at pH 9.76, temperature 34.69C, and an inoculum size of 9.51% (v/v),
respectively. A decolorization of 90.34% was observed with 100 mg L−1of Vat Brown R within 18h under these conditions.
Confirmatory experiments have verified the optimum combination of the three variables predicted by RSM. Kinetics study was
carried out using various approaches: Michaelis-Menten (Vmax = 29.1 mg L−1 h−1 and Km = 25.2 mg L−1), Lineweaver-Burk
(Vmax = 30.12 mg L−1 h−1 and Km = 26.91 mg L−1), and Eadie-hofstee model (Vmax = 30.23 mg L−1h−1and Km = 27.29 mg L−1),
and the results showed that the degradation followed a first-order reaction kinetics. The subsequent degradation of the dye and
the formation of metabolites were studied using analytical techniques such as UV-vis spectroscopy and FT-IR analysis. UV-vis
spectroscopy validated the detoxification of the dye and confirmed that Pseudomonas sp. NCH overcomes this decolorizing
activity through biodegradation. This study investigated the highest decolorization efficiency of strain NCH used in the
biodegradation of wastewaters containing anthraquinone dyes.
 
Date 2022-08-01T07:01:00Z
2022-08-01T07:01:00Z
2022-07
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60230
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.59(7) [July 2022]