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In vitro evaluation of free flavonoids in Cassia fistula Linn. calli of different ages under varying concentrations of plant growth regulators (PGRs)

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Title In vitro evaluation of free flavonoids in Cassia fistula Linn. calli of different ages under varying concentrations of plant growth regulators (PGRs)
 
Creator Kumar, Sushil
Vimala, Yerramilli
Singh, Ishwar
 
Subject Free flavonoids
Golden Shower
Indian Laburnum
Phenolics
Quercetin
Rutin
 
Description 635-639
Cassia fistula Linn, a paramount medicinal plant consumed by
huge population all over the world. Its bioactive metabolites
include flavonoids, phenolics, and polyphenols. Major objective
of current study is to find right media combination and age of
callus for maximum accumulation of free flavonoids in Cassia
fistula cultures. In this study, the calli were induced in MS + 1.0
mg/L 2,4-D + 0.05 mg/L BAP with 50 mg/L ascorbic acid. These
calli were then transferred to basic MS medium with 1.0 mg/L
2,4,5-T and varied BAP concentrations. Four and 8 weeks old
calli were used to find out the right concentration of BAP and age
for upscaling of free flavonoids. The samples were extracted and
HPTLC analyzed for free flavonoids at 260 nm along with the leaf
explants. The study revealed that the amount and number of free
flavonoids in 4 weeks old calli increased as the amount of
cytokinin (0.5 to 1.0 mg/L BAP) increased but decline with
further addition of BAP (2.0 mg/L BAP). In aged calli number
and amount of free flavonoids exhibit a tremendous increase when
compared to the calli in the same medium at 4 weeks. This is
inferred that accruing age of callus, reduced quantities of plant
growth regulators (optimized for callus growth) accumulate free
flavonoids. The best medium for free flavonoid production is MS
+ 1.0 mg/L 2,4,5-T with 1.0 mg/L BAP may be recommended for
producing a high amount of free flavonoids in C. fistula in vitro.
The results were justified with the results of quantification.
 
Date 2022-08-01T11:23:53Z
2022-08-01T11:23:53Z
2022-08
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/60236
https://doi.org/10.56042/ijeb.v60i08.64965
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.60(08) [AUG 2022]