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<p>Lactate dehydrogenase in fish spermatozoa and its role in sperm cell bioenergetics</p>

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Title Statement <p>Lactate dehydrogenase in fish spermatozoa and its role in sperm cell bioenergetics</p>
 
Added Entry - Uncontrolled Name Gronczewska, Jadwiga
Skorkowski, Edward Florian
 
Uncontrolled Index Term Adenylate concentration, Carp, Catfish, Cyprinus carpio, Clarias gariepinus, Clupea harengus, Herring, Tributyltin
 
Summary, etc. <p class="Abstract" style="text-align: justify;">Lactate dehydrogenase (LDH) in spermatozoa of three fish species was studied to determine its role in sperm cell bioenergetics in normal and polluted conditions. The adenylate energy charge (AEC) has been used as the measure of energy storage for adenine nucleotide pool of living cells and tributyltin (TBT) as a model toxicant. It was demonstrated that carp <em>Cyprinus carpio</em> Linnaeus, 1758 spermatozoa exhibit higher LDH activity than catfish <em>Clarias gariepinus</em> (Burchell, 1822) and herring <em>Clupea harengus</em> Linnaeus, 1758 spermatozoa. Native electrophoretic pattern of LDH from spermatozoa compared to somatic tissues extract shows nine LDH isoenzymes in carp spermatozoa, two LDH isoenzymes in herring and one LDH isoenzyme in catfish spermatozoa. It was confirmed that lactate plus pyruvate, which are the substrates for LDH seems to maintain AEC value. It indicates ATP synthesis in tricarboxylic acid cycle prevails upon the ATP utilization in catfish spermatozoa. It was noticed that LDH-B<sub>4</sub> is more strongly inhibited by TBT in herring than the nine LDH isoenzymes in carp spermatozoa which confirms that herring spermatozoa may be more sensitive for pollution by decreasing their energetic state. LDH role in maintaining physiological bioenergetic state of spermatozoa is also discussed.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-12-31 00:00:00
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/65331
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 01 (2020): IJEB [JANUARY 2020]
 
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