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<p style="text-align: justify;">A novel method for assessment of <em>in vitro </em>transcription for a bacterial promoter</p>

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Title Statement <p style="text-align: justify;">A novel method for assessment of <em>in vitro </em>transcription for a bacterial promoter</p>
 
Added Entry - Uncontrolled Name Uppal, Sheetal
Jawali, Narendra
Misra, Hari Sharan
 
Uncontrolled Index Term CyclicAMP Receptor Protein (CRP), IVT assay
 
Summary, etc. <p style="text-align: justify;"><em>In vitro</em> transcription (IVT) assay is a useful tool to monitor the transcriptional activity of a specific promoter under defined conditions <em>in vitro.</em> Conventional IVT assay involves use of radiolabeled probes which makes it tedious to perform and limits its utility for large scale applications. Here, a reverse-transcription-PCR (RT-PCR) based transcript detection method has been developed for bacterial <em>in vitro</em> transcription assay. Unlike conventional radiolabeling approach, this method is simple, fast, needs smaller reaction volumes, does not require special infrastructure and could be potentially used for any large-scale screening applications. The present study demonstrates the feasibility of this method by showing Cyclic AMP Receptor Protein (CRP) mediated activation of a CRP dependent <em>E. coli</em> promoter.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-12-31 00:00:00
 
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http://op.niscair.res.in/index.php/IJEB/article/view/65329
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 01 (2020): IJEB [JANUARY 2020]
 
Language Note en