Record Details

Pyramiding and evaluation of segregating lines containing lectin and protease inhibitor genes for aphid resistance in <em>Brassica juncea</em>

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Pyramiding and evaluation of segregating lines containing lectin and protease inhibitor genes for aphid resistance in <em>Brassica juncea</em>
 
Added Entry - Uncontrolled Name Jayaswal, Deepanshu
Mainkar, Pawan
Kumar, Kuldeep
Agarwal, Yamini
Kalia, Vinay
Kansal, Rekha
 
Uncontrolled Index Term Transgenics; Transgenic breeding; Chickpea lectin; Urdbean protease inhibitor
 
Summary, etc. Aphids are one of the most devastating pests, affecting the potential yield and quality ofBrassica juncea. In the current study, we have attempted to pyramid two transgenic lines containing chickpea lectin (CHPL, P1) and urdbean protease inhibitor (UPI, P2) in each under the phloem specific rolC promoter, through conventional breeding approach. In the derived F2 population, both lectin and protease inhibitor genes were segregating in a 9:3:3:1 ratio (p-value: 0.81), indicative of a single copy of the transgenes in the parents. Furthermore, the parental, as well as pyramided progenies were evaluated for their potential resistance to aphids in terms of mortality and natality. The lines containing both the transgenes were found to be superior over single gene transgenics as a higher mortality rate (96%) was found in F2on the 9th day as compared to single gene transgenics (86% and 80% in P1 and P2 respectively). A significant decrease in the number of nymphs was observed in P1 and P2 but most in F2 plants as almost 43, 32.08, and 107.5 times decrease in the number of nymphs was found in P1, P2, and F2 individuals over control. Expression profiling was done to see if there was any impact of gene pyramiding on the expression pattern of both transgenes before and after aphid treatment, and no significant changes were observed, indicating constitutive expression of transgenes in pyramided lines also. In conclusion, pyramided lines were found to be promising and were superior for aphid resistance.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2022-08-12 13:13:31
 
Electronic Location and Access application/pdf
application/pdf
http://op.niscair.res.in/index.php/IJBB/article/view/62319
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); IJBB Vol. 59 (8) [August 2022]
 
Language Note en