Record Details

<p>Phytochemical analysis, antimicrobial, antioxidant and enzyme inhibitory activities of ethanolic extract of<em>Centaureasolstitialis</em> L. and its different fractions</p>

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement <p>Phytochemical analysis, antimicrobial, antioxidant and enzyme inhibitory activities of ethanolic extract of<em>Centaureasolstitialis</em> L. and its different fractions</p>
 
Added Entry - Uncontrolled Name Ahmad, Saeed
Saba, Sahar
Hafiz, Muhammad Asif
 
Uncontrolled Index Term acetylcholinesterase, Antibacterial, Antifungal,Antimicrobial,butrylcholinesterase, Barnabys thistle, chymotrypsin, DPPH, golden star thistle, yellow star thistle
 
Summary, etc. Resistance to conventional antimicrobial regimes is one of the issues of concern in healthcare and it drives the need for development of new antimicrobial agents. Medicinal plants, as rich source of biochemical and bioactive compounds, serve as potential source for new drugs. Here, we evaluated the ethanolic extract of <em>Centaureasolstitialis</em>L.and its different fractions (n-hexane, choloroform and n-butanol soluble fraction) for antimicrobial, antioxidant and enzymes inhibitory activities. Antibacterial activity against two gram-positive and three gram-negative bacteria species were determined by using agar well diffusion and 96-wells microplate methods. Similarly, antifungal activity against two fungal strains was also evaluated by agar well diffusion method. Antioxidant activity analyzed by measuring the scavenging activity of DPPH radicals and acetylcholinesterase, butrylcholinesterase and chymotrypsin inhibitory activity was determined at 10 µg/mL and 1.0 mg/mL concentrations. Results revealed that the ethanolic extract of <em>C. solstitialis</em> and its different fractions possesses significant (<em>p</em>&lt;0.05) antibacterial activity and effective against fungi <em>Aspergillus niger</em>and<em>Macrophominaphaseolina</em>. Significant (<em>p</em>&lt;0.05) DPPH scavenging activity (88.52±0.23%) among all fractions was noted. n-butanol fraction showed significant acetyl-cholinesterase (78.55±0.76%) and butrylcholinesterase inhibitory activity (78.1±0.41%) with IC<sub>50 </sub>values of 54.6±0.39 µg/mL and 211.9±0.15 µg/mL, respectively. Maximum chymotrypsin inhibition activity was shown by crude ethanolic extract (87.76±1.17) with IC<sub>50</sub>valueof 38.23±0.75 µg/mL. It is concluded that <em>C. solstitialis</em> extract and its fractions possess significant antimicrobial, antioxidant and enzyme inhibitory activity
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-06-01 00:00:00
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/65503
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 06 (2020): IJEB [JUNE 2020]
 
Language Note en