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<p>meta-topolin and β-cyclodextrin enhance multiple shoot and root production in black gram <em>Vigna mungo</em> (L.) Hepper</p>

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Title Statement <p>meta-topolin and β-cyclodextrin enhance multiple shoot and root production in black gram <em>Vigna mungo</em> (L.) Hepper</p>
 
Added Entry - Uncontrolled Name Kapildev, Gnanajothi
Chinnathambi, Arunachalam
Sivanandhan, Ganeshan
Rajesh, Manoharan
Jeyaraj, Murugaraj
Selvaraj, Natesan
Alharbi, Sulaiman Ali
Ganapathi, Andy
 
Uncontrolled Index Term Aromatic cytokinin, β-CD, Cotyledonary node, Plant growth regulators, Ploidy
 
Summary, etc. <p class="Abstract" style="text-align: justify;">The recalcitrant nature of black gram is the major constraint of <em>in vitro </em>regeneration and <em>agrobacterium-</em> mediated genetic transformation, to overcome this, a productive shoot regeneration protocol has been achieved in black gram cultivar T9 using 7-day old cotyledonary node explants excised from <em>in vitro-</em> raised black gram seedlings using meta-topolin. An aromatic cytokinin,meta-topolin along with BA (1.5+0.5 mg/L) in 0.50 strength MS medium with 1.5% (w/v) sucrose exhibited a maximum number of multiple shoots (32.0±0.37 shoots/explant) at the end of 6 weeks of culture. The shoots were elongated in (6.40±0.50 cm/shoot) in MS medium supplemented with GA<sub>3</sub> (2.0 mg/L). A maximum number of roots (9.60±0.50/shoot) and root length (11.20±0.73 cm/shoot) were obtained in combination with β-cyclodextrin(a cyclic oligosaccharide;1.5 mg/L) and IBA (1.5 mg/L). The rooted plantlets were hardened and acclimatized with least mortality rate of 2% in pot mixture consisting red soil:sand:farm yard manure (FYM) (2:1:1) and grown in green house with 85% relative humidity. Ploidy levels were analyzed using flow cytometry which confessed the chromosomal stability in <em>invitro</em> raised plants similar to parent plants. This protocol may be useful for producing transgenic black gram with desirable agro-traits in Indian cultivars.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-05-01 00:00:00
 
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http://op.niscair.res.in/index.php/IJEB/article/view/65483
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 05 (2020): IJEB [MAY 2020]
 
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