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<p>Exploring prospective forested wetland — Actinomycetes for biodeterioration of genotoxic textile azo dyes</p>

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Title Statement <p>Exploring prospective forested wetland — Actinomycetes for biodeterioration of genotoxic textile azo dyes</p>
 
Added Entry - Uncontrolled Name Preethi, Sai
Pathy, Ramesh
 
Uncontrolled Index Term Aquatic pollution, Aerobic biotransformation, Biodecolourization, Bioremediation, Green gram, Molecular docking, Mung bean, Streptomyces coelicolor,Vignaradiata
 
Summary, etc. <p class="Abstract" style="text-align: justify;">disposal of synthetic textile dye effluents into waterbodies is a major cause of water pollution and undesirable toxicity on aquatic organisms affecting their metabolic and hormonal cycles. Moreover, it affects human health significantly by carcinogenic and mutagenic effects. Therefore, this study targets the biotreatment of azo dyes as a green eco-friendly tool using the tropical mangrove actinomycete, <em>Streptomyces coelicolor</em>strainSPR7, that manifests versatile decolourization of azo dyes. The highest decolourization rate (97.5%) was achieved using Methylene Blue (MB) within 72 h under the optimal conditions. Molecular docking analysis was accomplished by Autodock software, to discern docking score, binding pocket residues and interaction of azoreductase, DyP-type peroxidase and laccase with MB. The <em>in vitro</em> findings were highly correlating with <em>in silico</em> appraisals. UV-vis spectrometry, HPLC and FTIR were validating the MB biodecolourization and degradation. Interestingly, the strain detoxified MB <em>viz</em>. further validated by the <em>Vignaradiata</em> toxicity assay. Supplementarily, azo reductase was assessed to be the key biocatalyst for decolourization, while other two enzymes were involved in further mineralization of decolourized metabolites. Henceforth, a coupled in silico cum biological dye deterioration is a booming strategy for an economical, eco-friendly and time saving bioremediation to reuse textile wastewater.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-05-01 00:00:00
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/65486
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 05 (2020): IJEB [MAY 2020]
 
Language Note en
 
Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJEB/article/download/65486/465615983