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<p>MHC-<em>DRB</em>1 exon 2 polymorphism and its association with faecal egg count of <em>Haemonchus contortus</em> in Munjal sheep</p>

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Title Statement <p>MHC-<em>DRB</em>1 exon 2 polymorphism and its association with faecal egg count of <em>Haemonchus contortus</em> in Munjal sheep</p>
 
Added Entry - Uncontrolled Name Kumari, Anu
Kumar, Aman
Tomar, Prince
Baro, Daoharu
Grewal, Satyavir
Sangwan, ML
 
Uncontrolled Index Term Anthelmenthic resistance, GIT infection, Haemonchosis, Lambs, Livestock, Meat, PCR-RFLP
 
Summary, etc. Haemonchosis is an important disease of small ruminants. Anthelmenthic resistance has instigated the demand of other viable method for control of gastrointestinal parasite. Here, we investigated ovine major histocompatibility complex class II (Ovar MHC II) <em>DRB</em>1 exon 2 polymorphism and itsassociation with faecal egg count (FEC) of <em>Haemonchus contortus</em> in Munjal population of sheep. Genomic DNA was isolated from blood samples of 46 lambs between 6-7 months of age. The polymorphism in <em>DRB</em>1 gene was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. PCR products of exon 2 of <em>DRB</em>1 gene were digested with <em>Hae</em>II,<em> Bsa</em>HI and N<em>ci</em>I restriction enzymes. Fragment of the <em>DRB</em>1 gene comprising 9 bp of the 5' intron and 270 bp of entire exon 2 was successfully amplified. On digestion of 279 bp PCR product with N<em>ci</em>I, three genotypes <em>viz</em>. A1A1, A2A2 and A1A2 were found with allele frequencies 0.65 and 0.35. <em>Hae</em>II enzyme revealed three genotypes A1A1, A1A2 and A2A2 with allele frequencies of A1 and A2 were 0.42 and 0.58.<em>Bsa</em>HI enzyme also generated three genotypes A1A1, A1A2 and A2A2 with allele frequencies of A1 and A2 was 0.42 and 0.58. We were able to found polymorphism in <em>DRB</em>1 gene but no association could be established between genotypes generated by different restriction enzymes and FEC of <em>H. contortus</em> in Munjal sheep
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-05-01 00:00:00
 
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http://op.niscair.res.in/index.php/IJEB/article/view/65492
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 05 (2020): IJEB [MAY 2020]
 
Language Note en
 
Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJEB/article/download/65492/465616001