<p>Antiproliferative effects of total alkaloid extract of roots of <em>Chassaliacurviflora</em> (Wall.) Thwaites on cancer cell lines</p>
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Title Statement |
<p>Antiproliferative effects of total alkaloid extract of roots of <em>Chassaliacurviflora</em> (Wall.) Thwaites on cancer cell lines</p> |
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Added Entry - Uncontrolled Name |
Gopa, G Rajeswaril Lakshmi, S Nair, A Jayakumaran Gangaprasad, A Sudhakaran, PR Muraleedharan, D Nair, GM |
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Uncontrolled Index Term |
Alkaloids, Anticancer, anti-inflammatory, Apoptosis, chemotherapeutic, curved Flower Chasalis, folklore, immunomodulatory |
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Summary, etc. |
<em>Chassaliacurviflora</em> is used in folklore medicines for treating several ailments and infections owing to its anti-inflammatory properties. Though the plant has been reported to possess anti-inflammatory antihepatotoxic and analgesic activities, its anticancer potential has not been studied so far. In the present study, we investigated the antiproliferative effects of the total alkaloids isolated from the roots of <em>C. curviflora</em>.The total alkaloid was validated by MTT assay in three cancer cell lines, such as liver cancer cell line-A549, breast cancer cell line-MCF-7 and ovarian cancer cell line -HeLa. Significant antiproliferative effect (IC<sub>50 </sub>value 3.59±0.14*** µg/mL) was observed in A549 cells, and was taken for further studies. Cell cycle analysis showed that the cells got arrested in sub G0 phase and annexin V-FITC assay revealed that 27.<span style="text-decoration: line-through;">4</span>% cells were in early apoptosis and 7% cells in late apoptosis. The study revealed that the total alkaloids of <em>Chassaliacurviflora</em>roots possess significant antiproliferative and apoptotic activity |
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Publication, Distribution, Etc. |
Indian Journal of Experimental Biology (IJEB) 2020-06-01 00:00:00 |
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Electronic Location and Access |
application/pdf http://op.niscair.res.in/index.php/IJEB/article/view/65501 |
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Data Source Entry |
Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 06 (2020): IJEB [JUNE 2020] |
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Language Note |
en |
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