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<p>Isolation, cloning and characterization of <em>phl</em>B gene from an Indian strain of Gram negative soil bacteria <em>Pseudomonas fluorescens</em></p>

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Title Statement <p>Isolation, cloning and characterization of <em>phl</em>B gene from an Indian strain of Gram negative soil bacteria <em>Pseudomonas fluorescens</em></p>
 
Added Entry - Uncontrolled Name Dash, Prasanta K
Gupta, Payal
Panwar, Bhupendra Singh
Rai, Rhitu
 
Uncontrolled Index Term Antibacterial, Antifungal, DAPG, MAPG, Operon, PGPR, phlABCD, phloroglucinol
 
Summary, etc. <p class="Abstract" style="text-align: justify;">Phloroglucinol (PHL) or 2,4-diacetylphloroglucinol (DAPG) is a polyketide compound that exhibits broad spectrum anti-bacterial  and antifungal properties against soil-borne plant pathogens. It is produced by gram negative bacterium <em>Pseudomonas </em>(fluorescent <em>Pseudomonad</em>) which renders them a potential biological control agent. The process of PHL production is accomplished by a well coordinated activity of four (<em>phlABCD) </em>genes arranged in an operon. All four genes are essential and indispensable for biosynthesis of DAPG from precursor malonyl CoA. Here, we report cloning and analysis of <em>phlB </em>gene from an Indian strain of <em>Pseudomonas fluorescens</em>. Further, we predicted the structure of PHLB protein and analyzed its intrinsic details. Stereo-chemical properties were analyzed by Ramachandran plot and secondary structure was retrieved by PDB sum. Our study provides insight into the structure of PHLB protein and revealed that though <em>Pseudomonas </em>PHLB lacks conserved motifs and domains it is indispensable for monoacetyl phloroglucinol (MAPG) synthesis.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2020-06-01 00:00:00
 
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http://op.niscair.res.in/index.php/IJEB/article/view/65506
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 58, ##issue.no## 06 (2020): IJEB [JUNE 2020]
 
Language Note en