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Enhancement of bacteriorhodopsin production from novel haloarchaea strains of Marakanam region of Tamil Nadu

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Title Enhancement of bacteriorhodopsin production from novel haloarchaea strains of Marakanam region of Tamil Nadu
 
Creator Steffy, J
Abraham, L S
Inbakandan, D
Govindaraju, K
Dharani, G
 
Subject Bacteriorhodopsin
Elicitors
Haloarchaea
Purple membrane
Salt pan
 
Description 418-422
Haloarchaea from high saline habitats are known to contain industrially important value-added bioactive compounds
such as bacteriorhodopsin, carotenoids, lipids, and proteins. The diversity of Haloarchaea from geographically less explored
saline habitats has received less attention. In this study isolation and characterization of the biotechnological potential of
novel haloarchaea strains from solar saltern, a hypersaline environment in Marakanam, Tamilnadu was carried out. The
samples were collected during the pre-monsoon (July) and post-monsoon (January) season and ten different haloarchaea
strains were isolated. UV-Vis spectroscopy analysis revealed the bacteriorhodopsin production by all the isolated strains.
Further experiments were carried out to estimate the yield of bacteriorhodopsin and to assess their photocurrent activity and
biosensor applications. The maximum yield of bacteriorhodopsin was 5.6 mg/L of cultured lysate. This study will contribute
to a better understanding on effect of elicitors on bacteriorhodopsin producing different haloarchaea strains and growing
knowledge on their biotechnological applications.
 
Date 2022-08-22T11:48:04Z
2022-08-22T11:48:04Z
2022-05
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/60329
https://doi.org/10.56042/ijms.v51i05.65574
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJMS Vol.51(05) [May 2022]