Record Details

In-vitro biocompatibility studies of nanochitosan/ polyvinylpyrrolidone blends on MC3T3-E1 cells showing beneficial effect in bone tissue engineering

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title In-vitro biocompatibility studies of nanochitosan/ polyvinylpyrrolidone blends on MC3T3-E1 cells showing beneficial effect in bone tissue engineering
 
Creator Sangeetha, V
Sudha, P N
Jayaprabakar, J
 
Subject Cell viability
MTT assay
Nanochitosan
PVP
 
Description 396-403
Chitosan is a naturally occurring polymer with numerous biological properties that can be used in the biomedical field.
Chitosan was modified to nanochitosan in this study. The polycationic nature of chitosan interacts with polyanions (TPP) to
form nanoparticles. The prepared nanochitosan was mixed with polyvinyl pyrollidone to create binary blends with ratios of
1:1, 1:2, and 2:1. The polymeric blends were interpreted by using the analytical tools FTIR, XRD and SEM. The results of
the FTIR and XRD confirm the formation of binary blends with strong electrostatic interaction. The study aims to
investigate the compatibility of the prepared blend as tissue regenerative material. The blends were evaluated in-vitro using
fluorscent spectroscopy, MTT assay, NRR assay and ALP assay to study its biocompatibility and cell viability. The results
revealed that the quick multiplication followed by a moderate increment in cell number of MC3T3-E1 as cells reacted to the
prepared material and hence the materials can be used in the regenerative medicine.
 
Date 2022-08-22T11:56:33Z
2022-08-22T11:56:33Z
2022-05
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/60332
https://doi.org/10.56042/ijms.v51i05.65570
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJMS Vol.51(05) [May 2022]