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Association of bull semen protein estimates and SDS-PAGE profiles on semen freezability

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Title Association of bull semen protein estimates and SDS-PAGE profiles on semen freezability
 
Creator Alyethodi, Rafeeque R
Sirohi, Ajayvir Singh
Karthik, S
Tyagi, S
Sharma, Ankur
Muniswamy, K
Kundu, A
 
Subject Bradford Assay
Cryopreservation
Frieswal bulls
Sperm and seminal plasma proteins
SDS-PAGE
Semen
 
Description 854-859
It is vital to identify the ejaculate with good freezability by
determining the biochemical makeup of the ejaculate at the prefreeze
stage. The present study targeted to assess the use of the
protein estimates and profiles at the pre-freeze stage as markers of
freezability in Frieswal populations. Storing the proteins for
proteomic studies is always tricky in the case of animal studies,
where accessibility to liquid nitrogen is limited. Hence alternative
storing approaches need to be optimized. The second part of this
study examined the protein concentration and protein profiles of
RNALater and frozen stored sperm cells to assess the use of
RNALater preservation in sperm proteomic studies. Sperm and
seminal plasma protein concentrations were quantified using
Bradford assay, and total protein quantities were derived. The
seminal plasma and sperm protein profiles were generated with
SDS-PAGE. The protein estimates and SDS-PAGE profiles of
good and poor freeze-groups were similar. Also, sperm and
seminal plasma protein concentration were not correlated with the
semen volume and sperm count. Even though the yield was
comparatively less, the protein profiles of sperm preserved by
RNALater were similar to that of frozen sperms. The present
study results indicate that the protein estimates and qualitative
profiles of sperm and seminal plasma proteins may not be
sufficient to reveal the differences in the proteome of good and
poor freezable bulls at the macro level. Hence, the protein
estimates and profiles of neat semen may not be helpful for the
prediction of freezability at the pre-freeze stage. Secondly, this
study indicates that RNALater preservation helps store sperms for
proteome analysis studies.
 
Date 2022-08-23T11:05:49Z
2022-08-23T11:05:49Z
2022-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60338
https://doi.org/10.56042/ijbb.v59i8.60056
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.59(8) [AUGUST 2022]