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Pyramiding and evaluation of segregating lines containing lectin and protease inhibitor genes for aphid resistance in Brassica juncea

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Title Pyramiding and evaluation of segregating lines containing lectin and protease inhibitor genes for aphid resistance in Brassica juncea
 
Creator Jayaswal, Deepanshu
Mainkar, Pawan
Kumar, Kuldeep
Agarwal, Yamini
Prabha, Ratna
Kalia, Vinay
Kansal, Rekha
 
Subject Transgenics
Transgenic breeding
Chickpea lectin
Urdbean protease inhibitor
 
Description 800-807
Aphids are one of the most devastating pests, affecting the potential yield and quality ofBrassica juncea. In the current
study, we have attempted to pyramid two transgenic lines containing chickpea lectin (CHPL, P1) and urdbean protease
inhibitor (UPI, P2) in each under the phloem specific rolC promoter, through conventional breeding approach. In the derived
F2 population, both lectin and protease inhibitor genes were segregating in a 9:3:3:1 ratio (p-value: 0.81), indicative of a
single copy of the transgenes in the parents. Furthermore, the parental, as well as pyramided progenies were evaluated for
their potential resistance to aphids in terms of mortality and natality. The lines containing both the transgenes were found to
be superior over single gene transgenics as a higher mortality rate (96%) was found in F2on the 9th day as compared to single
gene transgenics (86% and 80% in P1 and P2 respectively). A significant decrease in the number of nymphs was observed in
P1 and P2 but most in F2 plants as almost 43, 32.08, and 107.5 times decrease in the number of nymphs was found in P1, P2,
and F2 individuals over control. Expression profiling was done to see if there was any impact of gene pyramiding on the
expression pattern of both transgenes before and after aphid treatment, and no significant changes were observed, indicating
constitutive expression of transgenes in pyramided lines also. In conclusion, pyramided lines were found to be promising
and were superior for aphid resistance.
 
Date 2022-08-23T11:26:58Z
2022-08-23T11:26:58Z
2022-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60345
https://doi.org/10.56042/ijbb.v59i8.62319
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.59(8) [AUGUST 2022]