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A bioinformatic approach towards designing a human papillomavirus vaccine based on L1 capsid protein sequence of HPV45

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Title A bioinformatic approach towards designing a human papillomavirus vaccine based on L1 capsid protein sequence of HPV45
 
Creator Karimah, Nihayatul
Sulfianti, Asri
Nurhasanah, Astutiati
 
Subject Bioinformatics
Consensus sequence
HPV45
Immune response
Vaccine design
 
Description 927-935
The cost of HPV vaccination is relatively expensive in low- to middle-income countries, hindering the introduction of
HPV vaccination in these areas, although infection cases are high. In this study we designed a vaccine candidate based on
L1 protein from Human Papillomavirus Subtype 45 (HPV45). Explorations of L1 HPV45 sequences from NCBI and
Uniprot databases generated a consensus sequence, which was then optimised to improve its antigenicity character, whilst
retaining the same epitope sites as observed in the consensus. Characteristics of the designed molecule was assessed, to
ascertain its potential immunogenicity and good physicochemical characters. The study showed no major difference
between our designed protein and either the Indonesian L1 HPV45 sequence (GenBank: QRG45832.1) (apart from two
amino acids, N379 and G383), or the consensus sequence (apart from three amino acids, N81, T329, and H392). These
differences do not seem to affect the 3D-structural similarities of the proteins. The designed protein is a non-allergenic,
60 kDa protein, with pI 8.61. It is relatively thermostable, with aliphatic index 75.26. The GRAVY score suggested that the
protein is soluble in water. Pichia was selected as the expression host, because, unlike in E. coli, the protein has longer halflife
and do not form inclusion bodies in the yeast.
 
Date 2022-08-30T08:26:04Z
2022-08-30T08:26:04Z
2022-09
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60401
https://doi.org/10.56042/ijbb.v59i9.62010
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.59(9) [SEP 2022]