Isolation and characterization of native Rhizobium from root nodules of raikia french bean growing area of Odisha
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Title |
Isolation and characterization of native Rhizobium from root nodules of raikia french bean growing area of Odisha
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Creator |
Verma, Himani
Patra, Ranjan Kumar Sethi, Debadatta Pattanayak, Sushanta Kumar |
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Subject |
Antibiotics
French bean Generation time Rhizosphere Stress tolerance |
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Description |
918-926
Injudicious application of nitrogenous fertilizers leads to soil quality deterioration which results into yield loss. The application of biofertilizer containing native efficient rhizobia enhances the nodular properties, N-fixation and soil quality. Therefore, fifty strains of Rhizobium were isolated from root nodule of Raikia french bean and among them only two isolates viz., RBHR-15 and RBHR-21 were confirmed as Rhizobium. The isolates were unable to grow under anaerobic conditions and failed to produce ketolactase enzyme, showed a negative response for gelatin liquefaction and Simmonâs citrate agar test, responded positively towards the indole test, MR-VP, TSI test and could produce NH4+ from peptone in thegrowth medium. The isolate RBHR-15, could reduce H2S and nitrate whereas, RBHR-21 could not. The growth of both isolates was luxuriant in the nutrient broth containing 1% NaCl and decreased with an increase in the concentration of NaCl and grew profusely in the pH range of 6-8. The generation time of RBHR-15 and RBHR-21 were 16.4and 10.6 h, respectively. The 16s rRNA of both isolates was sequenced and submitted to the National Center for Biotechnology Information (NCBI). The isolates RBHR-15, and RBHR-21 were assigned accession numbers MN480514 and MN480516. |
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Date |
2022-08-30T08:29:04Z
2022-08-30T08:29:04Z 2022-09 |
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Type |
Article
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Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60402 https://doi.org/10.56042/ijbb.v59i9.61519 |
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Language |
en
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Publisher |
NIScPR-CSIR, India
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Source |
IJBB Vol.59(9) [SEP 2022]
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