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Screening and molecular identification of marine sponges with cytotoxic activities, collected from Gulf of Mannar, Indian Ocean

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Title Screening and molecular identification of marine sponges with cytotoxic activities, collected from Gulf of Mannar, Indian Ocean
 
Creator Merlin, T S
Umar, M
Dhaneesha, M
Joseph, V
Sajeevan, T P
 
Subject Cytotoxic activity
Gulf of Mannar
Halichondria sp
NCI-H460
Rhabdastrella globostellata
Sponges
 
Description 529-535
The marine environment, a rich source of biological and chemical diversity, offers tremendous scope for discovering
novel metabolites with pharmacological activities. Marine invertebrates, especially sponges, have gained more attention in
drug discovery programmes since they exhibit unique metabolic and physiological capabilities and an extraordinarily
symbiotic association with diverse bacterial communities. The present study focuses on screening the cytotoxic activity of
the methanolic extracts of sponges against the Non-Small Cell Lung Cancer (NSCLC) cell line, NCI-H460. In the
bioactivity screening, two sponges (IOS-11 and IOS-12) were found with potent cytotoxic activity. The sponge IOS-12
showed almost complete cell growth inhibition (99 %) at 50 μg/ml and 80 % inhibition at 5 μg/ml; whereas IOS-11 showed
cell growth inhibition of 96 % and 77 % at 50 μg/ml and at 5 μg/ml, respectively. Amplification of the mitochondrial
cytochrome C oxidase subunit I (COI) and subsequent nucleotide analysis enabled the identification of sponge samples,
IOS-11 and IOS-12, as Rhabdastrella globostellata and Halichondria sp., respectively. The study concludes that the two
marine sponges, Rhabdastrella globostellata and Halichondria sp., possess excellent cytotoxic activities and could be
considered good candidates for the isolation of anticancer compounds with biomedical applications. The isolation,
purification and structural elucidation of the lead molecules from these sponges are ongoing studies, which might yield
potential new compounds with promising cytotoxic activities.
 
Date 2022-08-31T05:10:56Z
2022-08-31T05:10:56Z
2022-09
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/60417
https://doi.org/10.56042/ijms.v51i06.40629
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJMS Vol.51(06) [JUNE 2022]