Record Details

<em>Trametes versicolor</em> (L.) Lloyd as a source of thermostable serine protease: production and characterization

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement <em>Trametes versicolor</em> (L.) Lloyd as a source of thermostable serine protease: production and characterization
 
Added Entry - Uncontrolled Name Vishvakarma, Reena
Vimal, Archana
Mishra, Abha
Sharma, Poonam
Gaur, Vivek Kumar
 
Uncontrolled Index Term Basidiomycetes; Central Composite Design (CCD); Coriolus versicolor; Edible mushrooms; Plackett-Burman Design (PBD); Polypore mushroom; Polyporus versicolor; Response Surface Methodology (RSM); Solid-state fermentation; Trametes versicolor; Turkey tail
 
Summary, etc. Proteases are ubiquitously present and are among the largest groups of commercially important enzymes. Here, we investigated a wood-rot basidiomycete Trametes versicolor (L.) Lloyd [Syn. Coriolus versicolor (L.) Quél.; Polyporus versicolor (L.) Fr.] as a source of the enzyme serine protease, its production, and optimized to obtain a higher yield of the enzyme.. The significant variables with optimized values for maximum production of the enzyme were temperature (30C), incubation time (120 h) and wheat bran (10 g). The yield increased by 30.76% by statistically optimizing the media. The optimized temperature and pH for the maximum protease activity was 50C and pH 7.0, respectively. The enzyme was purified through ion exchange (using DEAE cellulose 52 resin) and gel filtration chromatography (using Superdex 200 column). The purified enzyme had a retention time of 7 min in RP-HPLC. The enzyme was stable at a broad range of temperature (30-60C) and pH (5.0-8.0) with a half-life of 58.72 min, Vmax of 37.17 μM min/mL and Km of 0.657 mg/mL. Its activity was enhanced by Na+, Ca2+, Mg2+ ions and SDS surfactant. These properties make this enzyme a valuable candidate for industrial applications
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2022-09-01 00:00:00
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/65147
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 60, ##issue.no## 09 (2022): IJEB [SEPTEMBER 2022]
 
Language Note en