Record Details

In vitro regeneration of plantlets from direct and indirect organogenesis from axenic stem nodal explant culture of a rare orchid Dendrobium jerdonianum Wight.

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title In vitro regeneration of plantlets from direct and indirect organogenesis from axenic stem nodal explant culture of a rare orchid Dendrobium jerdonianum Wight.
 
Creator Pyati, Ashok N.
 
Subject Dendrobium jerdonianum
nodal stem explants
PLBs
organogenic callus
regeneration
PGRs
 
Description 294-301
Direct and indirect in vitro plant regeneration protocol for Dendrobium jerdonianum has been established. Juvenile stem
nodal segments were used as explants to initiate the axillary buds/callus. Node explants derived from 7-8 months old in vitro
grown seedlings cultured on half strength Murashige & Skoog (MS) medium (½ MS) and supplemented with various plant
growth regulators (PGRs) viz., α-naphthalene acetic acid (NAA), 6-benzyl amino purine (BAP), kinetin (KN) and
thidiazuron (TDZ) either individually or in combinations produced axillary buds/callus. The highest average number of
shoots per explants were achieved in ½ MS medium supplemented with BAP (3.23 μM) induced the optimum response of
76.3% of direct shoots and forming an average number of 4.9 shoots per explant. Synergistic effects of combinations of
various PGRs on direct shoot induction were evaluated. The combinations of NAA (0.5 μM) + BAP (1.62 μM) and NAA
(0.5 μM) + KN (0.47 μM) induced 70.2 and 64.3% of direct shoots, respectively. On sub-culturing the shoots in the medium
containing NAA (2.69 μM) allows for further growth and rooting, developed into plantlets after 14 weeks of culture. The
stem nodal explants cultured on ½ MS medium supplemented with NAA (0.5 μM), TDZ (4.54 μM) and combination of
NAA (0.5 μM) + TDZ (2.27 μM) induced the optimum amount of organogenic callus of 39.6, 49.3 and 72.6%, respectively.
On sub-culturing the organogenic callus derived from the explants developed the protocorm like bodies (PLBs) in the same
media. These PLBs differentiated into plantlets after 18-21 weeks of culture. Almost 90% of the plantlets were successfully
acclimatized in the potting mixture and established ex vitro.
 
Date 2022-09-21T09:32:43Z
2022-09-21T09:32:43Z
2022-09
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/60519
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBT Vol.20(3) [July 2021]