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Efficient in vitro anther culture for androgenic plant production in F3-6 winter wheat (Triticum aestivum L.) bulk combinations

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Title Efficient in vitro anther culture for androgenic plant production in F3-6 winter wheat (Triticum aestivum L.) bulk combinations
 
Creator Kanbar, Osama Zuhair
Lantos, Csaba
Kiss, Erzsebet
Pauk, Janos
 
Subject Anther culture
androgenic traits
Triticum aestivum L.
genotype dependency
 
Description 284-293
The efficiency of in vitro anther culture (AC) method on six common wheat (Triticum aestivum L.) F3-6 bulk combinations
was investigated for the AC-induced androgenic traits, such as embryo-like structures (ELSs), regenerated plantlets, green
plantlets, albinos, transplanted plantlets, and acclimatized plantlets. Moreover, genotype dependency was tracked among the
tested combinations in the AC method. The results showed that all androgenic traits were produced in each tested combination
and non-significant differences were found among combinations for all androgenic traits except albinos. The number of green
plantlets ranged between 8.00 and 19.00 green plantlets/100 anthers based on the combination, with an overall mean of 14.37
green plantlets/100 anthers. The total number of acclimatized plantlets was 496 plantlets out of the 604 transplanted plantlets,
with a rate of 82.12%. This investigation confirms the efficiency and utility of in vitro AC method in winter wheat to produce
doubled haploid (DH) lines based on androgenesis for the breeding program and genetic studies. AC- induced DH lines
produced subsequently from acclimatized plantlet individuals in the nursery will be inserted in breeding programs for wheat
drought tolerance evaluation and in crossing programs for more improvement in green plants production in the nursery.
Albinism and genotype dependency, limiting-factors for wheat AC-induced DH production, were mitigated by the application
of some modifications of the wheat AC protocol. The modifications included a 2-week cold pre-treatment of donor tiller at 4C
in constant dim light and the use of the W14 mf induction medium instead of P4 mf.
 
Date 2022-09-21T09:37:16Z
2022-09-21T09:37:16Z
2022-09
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/60520
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBT Vol.20(3) [July 2021]