Record Details

A copper-zinc superoxide dismutase of Piper nigrum: Molecular cloning, recombinant expression, in vitro activity and molecular modeling

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title A copper-zinc superoxide dismutase of Piper nigrum: Molecular cloning, recombinant expression, in vitro activity and molecular modeling
 
Creator Reis, Sávio Pinho dos
Barros, Nicolle Louise Ferreira
Siqueira, Andrei Silva
Souza, Cláudia Regina Batista de
 
Subject Superoxide dismutase
black pepper
recombinant protein
heterologous expression
plant-pathogen interaction
 
Description 252-261
Superoxide dismutase (SOD) plays crucial role controlling oxidative burst produced during interactions of plants with
biotic factors. Previous studies identified a partial cDNA sequence coding for SOD in black pepper (Piper nigrum L.)
infected by Fusarium solani f. sp. piperis, causal agent of rot root disease. Here, our main aim was to isolate this full-length
cDNA sequence and characterize the P. nigrum SOD, named PnCuZnSOD. The 738-bp full-length cDNA contains a 459-bp
open reading frame (ORF) encoding a deduced protein with 152 amino acid residues, predicted to be a cytosolic protein
with molecular weight and isoelectric point of 15 kDa and 5.27, respectively. Comparative sequence analysis revealed high
identity between the deduced PnCuZnSOD and superoxide dismutase sequences from GenBank database. In addition,
putative conserved motifs, copper- and zinc-binding domains and cysteine residues involved in disulfide bond were
identified. Furthermore, molecular modeling analyzes generated the three-dimensional structure of PnCuZnSOD protein.
The recombinant PnCuZnSOD was produced by heterologous expression in bacterial cells, followed by the His-tagged
protein purification and enzymatic activity evaluation. The effects of heat and hydrogen peroxide on the PnCuZnSOD
activity were evaluated. The results indicated PnCuZnSOD as a probable active protein contributing to reactive oxygen
species (ROS) homeostasis in the cytosol of P. nigrum cells.
 
Date 2022-09-21T09:51:15Z
2022-09-21T09:51:15Z
2022-09
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/60523
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBT Vol.20(3) [July 2021]