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Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreak in Madhya Pradesh, India 2020

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Title Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreak in Madhya Pradesh, India 2020
 
Creator Gupta, Vandana
Nayak, Anju
Swamy, Madhu
Verma, Rupesh
Pandey, Megha
 
Subject lumpy skin disease virus
fusion gene; P32 gene
phylogenetic analysis
 
Description 236-241
Lumpy skin disease (LSD) is an emerging, economically important infectious viral disease of cattle caused by
Capripoxvirus. An outbreak of LSD has been reported in various states including Madhya Pradesh, India. This study was
undertaken to do molecular characterization of the lumpy skin disease virus (LSDV) and to study its phylogenetic
relationship with the sequences available in National Centre for Biotechnology Information (NCBI) database. A total of 6
blood samples and 2 tissue skin nodule samples were collected from cattle (aged from 6 months to 6 years) biopsies from
Bamhnoda & Sagda Jhapani village of Jabalpur District of Madhya Pradesh, India and were screened for LSDV by PCR.
The samples were subjected for amplification of P32 and fusion genes. Two tissue and three blood samples were positive for
P32 gene. Partial P32 and fusion genes were amplified and sequenced and subjected for phylogenetic analysis by MEGA6.
The phylogenetic analysis based on partial P32 gene and fusion gene showed close proximity with isolates from Egypt,
Saudi Arabia and China.
 
Date 2022-09-21T09:56:09Z
2022-09-21T09:56:09Z
2022-09
 
Type Article
 
Identifier 0972-5849 (print) 0975-0967 (online)
http://nopr.niscpr.res.in/handle/123456789/60525
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBT Vol.20(3) [July 2021]