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Molecular detection and genetic diversity of virulent Aeromonas hydrophila (Chester, 1901) Stanier, 1943 isolates from raw and processed fish

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Title Molecular detection and genetic diversity of virulent Aeromonas hydrophila
(Chester, 1901) Stanier, 1943 isolates from raw and processed fish
 
Creator Shaikh, M
Anjay
Kaushik, P
Kumar, A
Kumar, R
Sanjana
 
Subject Aeromonas hydrophila
AP-PCR
Fish
Virulence marker
 
Description 606-611
Aeromonas hydrophila is a mesophilic motile aeromonad present ubiquitously and causes a high number of fish
mortalities particularly in India along with various intestinal and extraintestinal diseases in humans. In this study, a total of
88 samples of fish and their processed products were screened by PCR targeting species-specific 16S rRNA gene, and the
virulent genes such as aerA, hly and ahh1. The findings of the study showed that a total of 36.36 % of samples harboured
A. hydrophila of which 51.61 %, 71.87 % and 78.12 % of isolates possessed virulent genes such as aerA, hly, and ahh1,
respectively. The phylogenetic analysis of the isolates using AP-PCR assay suggested that five different clusters of
organisms were prevalent among A. hydrophila with a high degree (> 95.00 %) of lineage. In conclusion, it may be
presumed that raw and processed fish harboured the pathogenic A. hydrophila which may serve as a reservoir/ source of
infection to human beings.
 
Date 2022-09-29T05:24:08Z
2022-09-29T05:24:08Z
2022-09
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/60557
https://doi.org/10.56042/ijms.v51i07.38166
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJMS Vol.51(07) [July 2022]