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Cultural, morphological and molecular diversity of Ustilaginoidea virens (Cooke) Tak. isolates causing false smut of rice in different agroclimatic regions of Karnataka

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Title Cultural, morphological and molecular diversity of Ustilaginoidea virens (Cooke) Tak. isolates causing false smut of rice in different agroclimatic regions of Karnataka
 
Creator AS, Savitha
A, Nagaraja
K, Ajithkumar
MK, Prasannakumar
 
Subject Chlamydospores
Disease severity
Growth characters
Oryza sativa
Paddy
 
Description 789-797
The ascomycete fungal pathogen Ustilaginoidea virens (Cooke) Takahshi cause false smut in rice and considerable yield
loss. In this study, we collected isolates of U. virens from the rice growing ecosystems of Karnataka and characterized for
cultural, morphological and molecular characters. The isolates of the fungus on Potato Sucrose Agar media exhibited
distinct colonies with colony growth ranging from 21.50 mm (Uv-20) to 70.00 mm (Uv-15). The colony colour appeared as
whitish to yellowish with varied growth pattern from flat, raised flat to fluffy and raised fluffy colonies with sectoring in
Uv-1, Uv-3, Uv-6 and Uv-9 isolates. The isolates of U. virens also showed variation in the morphology of spores, where the
conidia were globose, irregularly round to elliptical and warty on the surface with spore radius ranging from 2.91 to 5.36 μm.
The scanning electron microscopy revealed hyaline globose to irregularly rounded ornamented chlamydospores with
prominent spines. Besides cultural and morphological characters, molecular identification of false smut isolates was
confirmed through ITS sequencing which showed 91 to 99% identity with U. virens in NCBI-BLAST analysis. Dendrogram
constructed using ITS sequence data broadly separated the isolates into two major clusters with divergence among clusters.
This ITS (internal transcribed spacer) sequencing of isolates should help better understanding of the phylogenetic
relationships among these isolates.
 
Date 2022-09-29T08:54:03Z
2022-09-29T08:54:03Z
2022-09
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/60571
https://doi.org/10.56042/ijeb.v60i10.36997
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(10) [Oct 2022]