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Escherichia coli biofilm formation and control by phenolic compounds from Salvia officinalis L. extracts

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Title Escherichia coli biofilm formation and control by phenolic compounds from Salvia officinalis L. extracts
 
Creator Stefanović, Olgica
Mladenović, Dijana
Mladenović, Katarina
Grujović, Mirjana
Ivanović, Dragana
 
Subject Antimicrobials
Antibiofilm activity
Culinary sage
Garden sage
 
Description 771-780
Biofilm is recognized as one of the virulence factors of uropathogenic Escherichia coli. It offers bacteria significantly increased tolerance to antibiotics and makes difficulties in infection eradication. In this study, the biofilm formation ability of clinical isolates of uropathogenic E. coli as well as the antibiofilm activity of the common Garden sage, Salvia officinalis L.extracts were investigated in vitro. The influence of growth conditions (nutrient composition and incubation period) onbiofilm formation of E. coli strains was evaluated using crystal violet staining procedure. The tested strains better formedbiofilms after a longer incubation period (48 h). In addition, biofilm formation depended on nutrient medium composition.The eight strains were slime producers (Congo red agar assay). The water, ethanol, acetone and diethyl ether S. officinalisextracts were screened for their ability to inhibit the formation and metabolic activity of E. coli biofilms using crystalviolet and resazurin assay, respectively. The water extract was not active, while ethanol, acetone and diethyl etherextract exhibited antibiofilm activity (MBIC= 2.5-10 mg/mL). The microscopic visualization of treated E. coli LM1 biofilmhas shown morphological and density changes. In addition, the concentrations of phenolic compounds were determinedspectrophotometrically. The highest content of total phenolics was found in acetone extract while the flavonoids inwater extract.
 
Date 2022-09-29T08:59:04Z
2022-09-29T08:59:04Z
2022-09
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/60573
https://doi.org/10.56042/ijeb.v60i10.41435
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(10) [Oct 2022]