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<em>Escherichia coli </em>biofilm formation and control by phenolic compounds from <em>Salvia officinalis L.</em> extracts

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Title Statement <em>Escherichia coli </em>biofilm formation and control by phenolic compounds from <em>Salvia officinalis L.</em> extracts
 
Added Entry - Uncontrolled Name Stefanović, Olgica
Mladenović, Dijana
Mladenović, Katarina
Grujović, Mirjana
Ivanović, Dragana
 
Uncontrolled Index Term Antimicrobials; Antibiofilm activity; Culinary sage; Garden sage
 
Summary, etc. <p style="text-align: justify;">Biofilm is recognized as one of the virulence factors of uropathogenic <em>Escherichia coli</em>. It offers bacteria significantly increased tolerance to antibiotics and makes difficulties in infection eradication. In this study, the biofilm formation ability of clinical isolates of uropathogenic <em>E. coli </em>as well as the antibiofilm activity of the common Garden sage, <em>Salvia officinalis</em> <br /> L. extracts were investigated<em> in vitro</em>. The influence of growth conditions (nutrient composition and incubation period) on biofilm formation of <em>E. coli</em> strains was evaluated using crystal violet staining procedure. The tested strains better formed biofilms after a longer incubation period (48 h). In addition, biofilm formation depended on nutrient medium composition. The eight strains were slime producers (Congo red agar assay). The water, ethanol, acetone and diethyl ether <em>S. officinalis</em> extracts were screened for their ability to inhibit the formation and metabolic activity of <em>E. coli</em> biofilms using crystal <br /> violet and resazurin assay, respectively. The water extract was not active, while ethanol, acetone and diethyl ether <br /> extract exhibited antibiofilm activity (MBIC= 2.5-10 mg/mL). The microscopic visualization of treated <em>E. coli </em>LM1 biofilm has shown morphological and density changes. In addition, the concentrations of phenolic compounds were determined spectrophotometrically. The highest content of total phenolics was found in acetone extract while the flavonoids in <br /> water extract.</p><p style="text-align: justify;"> </p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2022-09-29 11:43:29
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/41435
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 60, ##issue.no## 10 (2022): IJEB [OCTOBER 2022]
 
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