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Effect of horseshoe crab perivitelline fluid on the viability of stem cells from human exfoliated deciduous teeth and expression of cell cycle regulatory

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Title Statement Effect of horseshoe crab perivitelline fluid on the viability of stem cells from human exfoliated deciduous teeth and expression of cell cycle regulatory
 
Added Entry - Uncontrolled Name Najian, I
Kannan, T P
Azlina, A
Khairani, I M
 
Uncontrolled Index Term Cell proliferation, Gene expression, Horseshoe crab, Perivitelline fluid, Stem cells from human exfoliated deciduous teeth
 
Summary, etc. <p>The effect of perivitelline fluid (PVF) (0.019 mg/ml) on the stem cell viability extracted from human exfoliated deciduous teeth was assessed using LIVE/DEAD viability/cytotoxicity. It showed that PVF enhanced viability as observed by higher live cell percentage and a greater number of viable cells in the treated group for 3 days. The expression of selected cell cycle regulatory genes investigated using reverse transcriptase polymerase chain reaction (RT-PCR) for 21 days showed that <em>CDKN2A, PTEN</em> and <em>TP53</em> expressed significantly higher in the treated group, suggesting that PVF enhanced SHED growth and proliferation. <em>MDM2</em> expression remained at low levels in the treated group indicating that PVF did not result in tumorigenic growth. Low expression of apoptotic activator gene, <em>BCL2L11 </em>in treated group from day 1 until 14 with a sudden peak on day 21 was noted. This is deemed normal as the cells thrive to maintain homeostatic level at the 21<sup>st</sup> day of incubation.</p>
 
Publication, Distribution, Etc. Indian Journal of Geo-Marine Sciences (IJMS)
2022-09-30 00:10:20
 
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http://op.niscair.res.in/index.php/IJMS/article/view/66099
 
Data Source Entry Indian Journal of Geo-Marine Sciences (IJMS); ##issue.vol## 50, ##issue.no## 02 (2021)
 
Language Note en