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Anti-proliferating effect of Ocimum sanctum and Centella asiatica plant extract on growth of human glioblastoma cells: An in vitro study

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Title Anti-proliferating effect of Ocimum sanctum and Centella asiatica plant extract on growth of human glioblastoma cells: An in vitro study
 
Creator Biswas, Biplob K
Beg, Mirza Masroor Ali
Samadhiya, Amit
Jamatia, Eliva
Gowda, Srinivas H
 
Subject Anticancer
Cytotoxicity
Eugenol
Medicinal plant
Survivin
U87MG
 
Description 956-962
Glioblastoma is aggressive brain tumour with poor prognosis with conventional chemotherapy, hence there is need to
find alternative targets for developing newer treatment. Advent of new treatment methods involving medicinal plants have
shown to reduced Cancer mortalities and prevents development of drug resistance for chemotherapy. Present study aimed at
investigates the anti-proliferating activity of two promising medicinal plants, Ocimum sanctum and Centella asiatica. We
studied the effect of their plant extract on U87MG Glioblastoma cells proliferation, survival effect and apoptosis. Cytotoxic
activity was assessed, after the plant extract treatment on U87MG using MTT assay with dose of 1 mg/mL to 25 mg/mL and
apoptosis assess was done using Annexin V assay with the three dose (1.5 mg/mL, 2 mg/mL and 2.5 mg/mL). Survivin gene
expression was studied using QRT-PCR (Rotar gene Q, Qiagen) has a marker of proliferation. Ocimum sanctum and
Centella asiatica treatment of U87MG cells with dosage of 1.5 mg/mL, 2.0 mg/mL, 2.5 mg/mL showed increase in mean
apoptotic cells 2.8 %, 4.9%, 10 % and 3.1%, 5.8% and 7.2%, respectively, compared to untreated U87MG cells. Survivin
gene analysis of U87MG cells showed down-regulation in gene expression and differences was significant in comparison to
untreated control group with both the plant extract, Centella asiatica showed more down-regulation (97% with 2.5 mg/mL)
than Ocimum sanctum. Ocimum sanctum and Centella asiatica exhibited promising anti-proliferating activity and induces
apoptosis by down regulation of survivin gene expression.
 
Date 2022-10-06T10:00:17Z
2022-10-06T10:00:17Z
2022-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60644
https://doi.org/10.56042/ijbb.v59i10.38606
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.59(10) [Oct 2022]