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Isolation and identification of thermo stable multi catalytic Bacillus licheniformis strain V7 from Ganeshpuri hot spring

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Title Isolation and identification of thermo stable multi catalytic Bacillus licheniformis strain V7 from Ganeshpuri hot spring
 
Creator Girdhari, Sonali
Pathak, Anupama P
 
Subject Ganeshpuri
Hot spring
Multi enzyme
Physiochemical analysis
Thermostable
 
Description 356-361
An efficient extracellular multi enzyme producer strain was isolated from Ganeshpuri hot spring located near
Vajreshwari in Thane district Maharashtra. It was identified by the cultural, biochemical, MALDI-TOF MS analysis and
16sRNA sequencing as strain of Bacillus licheniformis. It was isolated at the incubation temperature of ≥ 50ºC and showed
luxuriant growth and enzyme production ability in the similar temperature range. The multi enzyme producer strain was
checked for amylase, mannanase, pectinase, lipase, and cellulase production ability. Its hydrolytic index was measured by
taking the ratio of zone of substrate clearance to the colony size diameter. The observed hydrolytic index for cellulase was 5,
for pectinase 2.75, for lipase 1.4 after incubation at 52°C for 72 h. Similarly observed hydrolytic index for mannanase was
2.8 and for amylase 3.5 after incubation at 52°C for 24 h. The study resulted in isolation of an efficient thermo stable multi
enzyme producer strain of Bacillus licheniformis from Ganeshpuri hot water spring which encourages future studies to
explore various whole cell and enzyme applications.
 
Date 2022-10-18T06:20:48Z
2022-10-18T06:20:48Z
2022-10
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/60679
https://doi.org/10.56042./ijnpr.v13i3.38114
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.13(3) [September 2022]