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Development of a Cell Culture System from Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider)

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Relation http://eprints.cmfri.org.in/5719/
 
Title Development of a Cell Culture System from Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider)
 
Creator Sobhana, K S
George, K C
Venkat Ravi, G
Ittoop, Gijo
Paulraj, R
 
Subject Biochemistry
 
Description A cell culture system was developed successfully from gill explants of the Malabar grouper,
Epinephelus malabaricus. Gill tissue samples aseptically excised from healthy juveniles of
E. malabaricus were explanted in Leibovitz’ L-15 medium supplemented with 0.07 M NaCl and
20% fetal bovine serum (FBS). A mixture of different types of cells emerged from the explants,
and these cells were observed to spread and attach to the culture flasks from the second day
onwards. Confluent monolayers comprising epithelioid as well as fibroblast-like cells were formed
within ten days. The cells were found to grow well at 28 + 2°C. The cell monolayers were
subcultured by trypsinization and seeded into new flasks, which produced confluent monolayers
comprising predominantly epithelioid-like cells in subsequent passages.
 
Publisher Asian Fisheries Soceity; Malaysia
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/5719/1/3.pdf
Sobhana, K S and George, K C and Venkat Ravi, G and Ittoop, Gijo and Paulraj, R (2009) Development of a Cell Culture System from Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider). Asian Fisheries Science, 22 (2). pp. 541-547.