Purification, Characterization and Application Study of Bacterial Tannase for Optimization of Gallic acid Synthesis from Fruit Waste
NOPR - NISCAIR Online Periodicals Repository
View Archive InfoField | Value | |
Title |
Purification, Characterization and Application Study of Bacterial Tannase for Optimization of Gallic acid Synthesis from Fruit Waste
|
|
Creator |
Dhiman, Sunny
Mukherjee, Gunjan Kumar, Anu Majumdar, Rita Singh |
|
Subject |
Bacillus haynesii
Enzyme Hydrolysis Quantification Yield |
|
Description |
1029-1036
Tannase produced extracellularly by the bacterial strain Bacillus haynesii SSRY4 MN031245 was purified in step-wise manner through ammonium sulphate precipitation, dialysis, followed by anion exchange chromatography. Tannase was purified to 42.0-fold with 36.30% enzyme yield. The enzyme was relatively stable from 30 to 50℃ and pH (4.0–6.0) for up to 4 hours. Partially purified tannase (16.80 U/ml) was able to synthesize 20.304 mg/ml gallic acid from the fruit waste under optimized conditions. The results of application study suggest that bacterial tannase could provide a new source for Gallic acid synthesis from the fruit waste for industrial applications. Our research findings could provide a value chain to fruit waste and help in reducing the waste generation from fruit processing industries. |
|
Date |
2022-10-28T09:51:55Z
2022-10-28T09:51:55Z 2022-10 |
|
Type |
Article
|
|
Identifier |
0022-4456 (Print); 0975-1084 (Online)
http://nopr.niscpr.res.in/handle/123456789/60747 https://doi.org/10.56042/jsir.v81i10.55236 |
|
Language |
en
|
|
Publisher |
NIScPR-CSIR, India
|
|
Source |
JSIR Vol.81(10) [Oct 2022]
|
|