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Purification, Characterization and Application Study of Bacterial Tannase for Optimization of Gallic acid Synthesis from Fruit Waste

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Title Purification, Characterization and Application Study of Bacterial Tannase for Optimization of Gallic acid Synthesis from Fruit Waste
 
Creator Dhiman, Sunny
Mukherjee, Gunjan
Kumar, Anu
Majumdar, Rita Singh
 
Subject Bacillus haynesii
Enzyme
Hydrolysis
Quantification
Yield
 
Description 1029-1036
Tannase produced extracellularly by the bacterial strain Bacillus haynesii SSRY4 MN031245 was purified in step-wise
manner through ammonium sulphate precipitation, dialysis, followed by anion exchange chromatography. Tannase was
purified to 42.0-fold with 36.30% enzyme yield. The enzyme was relatively stable from 30 to 50℃ and pH (4.0–6.0) for up
to 4 hours. Partially purified tannase (16.80 U/ml) was able to synthesize 20.304 mg/ml gallic acid from the fruit waste
under optimized conditions. The results of application study suggest that bacterial tannase could provide a new source for
Gallic acid synthesis from the fruit waste for industrial applications. Our research findings could provide a value chain to
fruit waste and help in reducing the waste generation from fruit processing industries.
 
Date 2022-10-28T09:51:55Z
2022-10-28T09:51:55Z
2022-10
 
Type Article
 
Identifier 0022-4456 (Print); 0975-1084 (Online)
http://nopr.niscpr.res.in/handle/123456789/60747
https://doi.org/10.56042/jsir.v81i10.55236
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source JSIR Vol.81(10) [Oct 2022]