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Synthesis of zinc oxide nanoparticles, its characterization and anti-microbial activity assessment

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Title Synthesis of zinc oxide nanoparticles, its characterization and anti-microbial activity assessment
 
Creator Khosla, Saarthak
Singh, Priyanka
Malik, Mansi
Srivastava, Saurabh Kumar
Verma, Anita Kamra
 
Subject Bacterial resistance
DNA fragmentation assay
Kirby-Bauer method
ZnO NPs
 
Description 1106-1112
Zinc oxide nanoparticles (ZnO NPs) are one of the most abundant metal oxides nanoparticles. It provides excellent
thermal, electrical and chemical stabilities with low biotoxicity; its photo-oxidising and photo-catalytic impact on biological
and chemical species is of great importance, thereby making it a promising candidate to be used for in vitro and in vivo
studies in biomedical field. Hereby, ZnO NPs were synthesized using precipitation method with zinc acetate and sodium
hydroxide as starting materials. This study has characterized the synthesized ZnO NPs using different techniques such as
UV-Visible spectroscopy indicating a peak at 365 nm wavelength, size of ZnO NPs was determined to be 286.7 nm by
measuring hydrodynamic radii using Dynamic Light Scattering (DLS) phenomena. Further predominant charge existing at
surface of the synthesised ZnO NPs was evaluated to be 31.6 mV. Anti-microbial activity of ZnO NPs was determined by
Kirby-Bauer method for both Gram-positive and Gram-negative bacteria, S. aureus and E. coli, respectively. Anti-microbial
activity was determined as Zone of Inhibition that measures both bactericidal and bacteriostatic activity of ZnO NPs and was
found to be more potent for Gram-positive (S. aureus) bacteria and its activity increased with increasing concentration of
nanoparticles. Growth kinetics was studied to determine percentage growth inhibition, for this optical density was recorded
as a function of time in bacterial culture broth with and without treatment. Further DNA fragmentation assay was performed
to determine genotoxicity caused by nanoparticles and its effect on genomic DNA of bacteria. Highlighting its potential role
as a nano-carrier system for leading antibacterial drugs for enhanced effectiveness of the antibacterial therapies.
 
Date 2022-11-07T08:27:05Z
2022-11-07T08:27:05Z
2022-11
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60803
https://doi.org/10.56042/ijbb.v59i11.67313
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.59(11) [November 2022]