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<p><em>In vitro</em> Direct Regeneration and <em>Agrobacterium Tumefaciens</em> mediated <em>in planta</em> Transformation of <em>Ocimum sanctum L.</em></p>
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| Authentication Code |
dc |
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| Title Statement |
<p><em>In vitro</em> Direct Regeneration and <em>Agrobacterium Tumefaciens</em> mediated <em>in planta</em> Transformation of <em>Ocimum sanctum L.</em></p> |
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| Added Entry - Uncontrolled Name |
Khan, Sana ; CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow 226 015, Uttar Pradesh, India Husain, Zakir ; CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow 226 015, Uttar Pradesh, India Rahman, Laiq ur; CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow 226 015, Uttar Pradesh, India CSIR-CIMAP |
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| Uncontrolled Index Term |
Gus-A, Kanamycin, MAPs, Npt-II, O. tenuiflorum, Petiole |
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| Summary, etc. |
<p>An <em>in vitro</em> regeneration system for propagation has been successfully developed for a valuable medicinal and aromatic plant ‘<em>Ocimum sanctum</em> L’. In the present study, petiole explants, from <em>in-vitro</em> grown cultures of <em>O. sanctum,</em> was used for direct regeneration. The developed protocol employed 98% of regeneration frequency in addition to 9.6 shoots per explant when cultured on Murashige and Skoog (MS) medium fortified with 3 mg/L benzylamino purine (BAP) and 1 mg/L Naphthalene acetic acid (NAA). Furthermore, <em>Agrobacterium tumefaciens </em>Mediated genetic Transformation (ATMT) protocol (transient and stable) was also developed using LBA4404 strain harboring pBI121 with <em>uid-A</em> and neomycin phosphotransferase genes. The regenerated transformants were shifted on MS with kanamycin (50 mg/L) and afterwards placed on the half-strength MS medium. The validation was done through polymerase chain reaction (PCR) with neomycin phosphotransferase-II (<em>npt-II)</em> & β-glucoronidase (<em>uid-A)</em> gene primers. The maximum stable transformation frequency of 70% ± 0.35 was achieved. Hence, it is apparent that the established protocols <em>i.e.</em> <em>in vitro</em> direct regeneration and ATMT are appropriate for integrating novel enzymes/genes through high throughput techniques such as gene tagging, and targeted gene replacement to modulate the primary as well as secondary metabolic flux towards desired agronomic product <br /> or trait <em>in planta.</em><strong> </strong></p><div><em><br /></em></div> |
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| Publication, Distribution, Etc. |
Journal of Scientific & Industrial Research 2022-11-13 06:46:16 |
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| Electronic Location and Access |
application/pdf http://op.niscair.res.in/index.php/JSIR/article/view/59665 |
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| Data Source Entry |
Journal of Scientific & Industrial Research; ##issue.vol## 81, ##issue.no## 10 (2022): Journal of Scientific & Industrial Research |
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| Language Note |
en |
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| Nonspecific Relationship Entry |
http://op.niscair.res.in/index.php/JSIR/article/download/59665/465595603 http://op.niscair.res.in/index.php/JSIR/article/download/59665/465595606 http://op.niscair.res.in/index.php/JSIR/article/download/59665/465595607 http://op.niscair.res.in/index.php/JSIR/article/download/59665/465595610 http://op.niscair.res.in/index.php/JSIR/article/download/59665/465595611 http://op.niscair.res.in/index.php/JSIR/article/download/59665/465595613 |
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