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<p>Statistical Optimization Approaches for High Cell Biomass Production of <em>Lactobacillus casei</em></p>

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Title Statement <p>Statistical Optimization Approaches for High Cell Biomass Production of <em>Lactobacillus casei</em></p>
 
Added Entry - Uncontrolled Name Eyahmalay, J ; Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Johor 81310, Malaysia SBG Agrifeed SDN BHD, PLO369, Jalan Emas Dua, Kawasan Perindustrian Pasir Gudang, 81700, Pasir Gudang, Johor Bahru, Malaysia School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
Elsayed, E A; Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia Natural and Microbial Products Department, National Research Centre, Dokki, Cairo 12311, Egypt
Dailin, D J; Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Johor 81310, Malaysia School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
Ramli, S ; Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Johor 81310, Malaysia
Sayyed, R Z; Department of Microbiology, PSGVP Mandal’s, Arts, Science, and Commerce College, Shahada 425 409, Maharashtra, India
El-Enshasy, H A; Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Johor 81310, Malaysia School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria 21934, Egypt
 
Uncontrolled Index Term Box-Behnken design, High cell biomass, Lactobacillus casei, Medium optimization, Statistical optimization
 
Summary, etc. <p>Probiotic bacteria are known to treat and prevent diseases and hence promote physical and mental wellness due to their significant brain-gut relationship. The main challenge involved in probiotic commercialization is the bio processing limitation to produce high cell mass, especially with the cultivation of lactic acid bacteria which produces lactic acid as a by product. Synthesis of lactic acid by lactic acid bacteria inhibits bacterial growth, and in turn disrupts high cell mass production. Current work presents the findings for <em>Lactobacillus casei</em> medium optimization by response surface methodology in shake flask level. A simple medium using 4 components: lactose, soybean meal, yeast extract and magnesium sulphate has been identified to produce high cell mass than generic mediaused for probiotic cultivation, such as the MRS medium. Secondly, response surface methodology using Box-Behken Design was employed as an optimization strategy. After optimization process, the production of <em>Lactobacillus casei</em> biomass increased by about 164.6% recording 6.51g.L<sup>-1</sup> compared to cell biomass obtained using initial un-optimized medium (2.46g.L<sup>-1</sup>).</p>
 
Publication, Distribution, Etc. Journal of Scientific & Industrial Research
2022-11-19 05:33:33
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/JSIR/article/view/68645
 
Data Source Entry Journal of Scientific & Industrial Research; ##issue.vol## 79, ##issue.no## 3 (2020): Journal of Scientific & Industrial Research
 
Language Note en