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Title A Proteogenomic Analysis of Haptoglobin in Malaria
 
Names AWASTHI, G
TYAGI, S
KUMAR, V
PATEL, SK
ROJH, D
SAKRAPPANAVAR, V
KOCHAR, SK
TALUKDAR, A
SAMANTA, B
DAS, A
SRIVASTAVA, S
PATANKAR, S
Date Issued 2018 (iso8601)
Abstract ScopeHaptoglobin (Hp), an acute phase inflammatory protein is associated with malaria pathogenesis in several proteomics and genomics studies. The Hp gene has two co-dominant alleles: Hp1 and Hp2 that produce three genotypes: Hp1/Hp1, Hp1/Hp2 and Hp2/Hp2. Experimental designIn this study, validation of the proteomics data with Multiple Reaction Monitoring Mass Spectroscopy (MRM-MS) is performed and the association of the Hp gene variants with severe, non-severe malaria and community (healthy) controls using genotyping PCRs and DNA sequencing is analysed. ResultsHighly significant values of Hp is observed in the MRM assay that show a correlation with severity of malaria and is clearly distinguished from another febrile disease, dengue. Moreover, the Hp2/Hp2 genotype is seen in high percentages in non-severe malaria patients (74%) and community controls (72%) whereas patients diagnosed with severe malaria show only (31%) of this genotype. Sequencing of the Hp promoter region reveals three SNPs along with 10 unique haplotypes, out of which five are associated with non-severe and three with severe malaria populations ((2)=130; df=18; p<0.0001). Conclusion and clinical relevanceThis proteo-genomic study focuses on the correlation of the Hp protein and gene with malaria, thus highlighting the pivotal role of this acute phase immune gene in malaria pathogenesis.
Genre Article; Proceedings Paper
Topic alleles
Identifier PROTEOMICS CLINICAL APPLICATIONS,12(4)