| Abstract |
Pseudomonas sp. strain PP4 and C5 utilize phthalate isomers (o-, m- and p-) and carbaryl as carbon source, respectively. Degradative pathways were elucidated by isolating and characterizing metabolites, whole-cell O-2 uptake and enzyme activity studies. Metabolic studies suggest that phthalate isomer degrading pathways converge at 3,4-dihydroxybenzoic acid. Phthalate dioxygenases responsible for the degradation of the respective phthalate isomers are induced specifically, suggesting that probably there are three different phthalate dioxygenases. This was supported by whole-cell O-2 uptake studies and cells grown on glucose failed to show the activity of phthalate pathway enzymes. Glucose-grown cells lost the phthalate degradation property indicating probable involvement of plasmid, which is expressed and maintained selectively in the presence of phthalate isomers. The metabolic studies with Pseudiomonas sp. strain C5 suggest that carbaryl is first hydrolyzed to 1-naphthol by carbaryl hydrolase. Generated 1-naphthol is metabolized via 1,2-dihydroxynaphahtlane, salicylate and gentisic acid to TCA cycle intermediates, thus serving as the sole source of carbon and energy. The ability to utilize phthalates (0.3%) and carbaryl (1%) at high concentrations make these strains suitable candidates for bioremediation. Detailed understanding of metabolic pathways and genetic make-up will enable one to modify these strains by genetic engineering tools for suitable application in bioremediation. |
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