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| Field | Value |
| Title | A low voltage single cell electroporator with a microfabricated sense-porate aperture |
| Names |
SARKAR, A
MITRA, B SHASTRY, A WADIA, S MULHERKA, R LAL, R |
| Date Issued | 2004 (iso8601) |
| Abstract | We present a novel flow-type single cell electroporation (SCE) system for low voltage electroporation of biological cells. We have used a microfabricated silicon sense-porate aperture to detect and identify a cell by its impedance and then apply the optimum electric field on it. Incorporation of a fluorescent dye into mouse embryo fibroblast NIH-3T3 cells has been demonstrated with a cell survival rate or viability much higher than conventional macroelectroporators. This paper reports the principle, design and implementation of this system and the experimental results obtained using it. |
| Genre | Proceedings Paper |
| Identifier | MEMS 2004: 17TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST,375-378 |