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Biofabrication and optimization of silver nanoparticles using Campsis sp. to explore their antimicrobial properties

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Title Biofabrication and optimization of silver nanoparticles using Campsis sp. to explore their antimicrobial properties
 
Creator Bharadwaj, Kriti
Jha, Bhavana Sharma
Kathpalia, Renu
 
Subject Biofabrication
Campsis sp.
DLS
Silver Nanoparticles
Zeta potential and ZOI (Zone of Inhibition)
 
Description 1176-1183
Plant mediated green synthesis of silver nanoparticles (AgNPs) holds promising applications in the field of Biomedicine, Food
packaging and Wound healing. In the present investigation, biofabrication of AgNPs was performed using the aqueous extracts of
Campsis sp. (Family Bignoniaceae) leaves and flowers growing in the premises of Kirori Mal College, University of Delhi, Delhi.
Optimization of AgNPs was performed to analyse the varying effect of pH (6.0, 8.0, 10.0) and silver salt concentration (2 mM,
4 Mm and 6 Mm) in controlling the shape and size of AgNPs which in turn governs their further applications. Interestingly, change
in colour of the reaction mixture from pale yellow to reddish brown indicated the formation of AgNPs. These AgNPs were further
characterized by UV-Visible spectroscopy and showed peak in the range of 400-450 nm which confirmed the synthesis of silver
nanoparticles. Dynamic light scattering and zeta potential analysis (DLS-Zeta) confirmed the size of AgNPs around 200-300 nm.
A significant zone of inhibition was observed for both Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative)
bacterial strains which revealed the antimicrobial potential of Campsis sp. AgNPs. Therefore, Campsis AgNPs may provide a
green, eco-sustainable alternate method for sustainable production of nanomaterials for biomedical applications. These AgNPs may
also show tremendous applications in food packaging, wound healing and biomedical fields.
 
Date 2022-11-30T11:31:55Z
2022-11-30T11:31:55Z
2022-11
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/60975
https://doi.org/10.56042/ijbb.v59i12.67299
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.59(12) [December 2022]